Sandwich mode lateral flow assay for point-of-care detecting SARS-CoV-2

被引:15
|
作者
Pei, Fubin [1 ,2 ]
Feng, Shasha [1 ,2 ]
Hu, Wei [2 ]
Liu, Bing [2 ]
Mu, Xihui [2 ]
Hao, Qingli [1 ]
Cao, Yang [1 ]
Lei, Wu [1 ]
Tong, Zhaoyang [2 ]
机构
[1] Nanjing Univ Sci & Technol, Sch Chem & Chem Engn, Nanjing 210094, Jiangsu, Peoples R China
[2] State Key Lab NBC Protect Civilian, Beijing 102205, Peoples R China
基金
中国国家自然科学基金;
关键词
Lateral flow assay; SARS-CoV-2; Immunoassay; Point-of-care; RAPID DETECTION; IGG ANTIBODY; DIAGNOSIS; STRIP; NANOPARTICLES; BIOSENSOR; EMISSION; COVID-19; PROTEIN;
D O I
10.1016/j.talanta.2022.124051
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The global corona virus disease 2019 (COVID-19) has been announced a pandemic outbreak, and has threatened human life and health seriously. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), as its causative pathogen, is widely detected in the screening of COVID-19 patients, infected people and contaminated sub-stances. Lateral flow assay (LFA) is a popular point-of-care detection method, possesses advantages of quick response, simple operation mode, portable device, and low cost. Based on the above advantages, LFA has been widely developed for detecting SARS-CoV-2. In this review, we summarized the articles about the sandwich mode LFA detecting SARS-CoV-2, classified according to the target detection objects indicating genes, nucleo-capsid protein, spike protein, and specific antibodies of SARS-CoV-2. In each part, LFA is further classified and summarized according to different signal detection types. Additionally, the properties of the targets were introduced to clarify their detection significance. The review is expected to provide a helpful guide for LFA sensitization and marker selection of SARS-CoV-2.
引用
收藏
页数:21
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