Insight into structural characteristics of theabrownin from Pingwu Fuzhuan brick tea and its hypolipidemic activity based on the in vivo zebrafish and in vitro lipid digestion and absorption models

被引：26

作者：

Xiao, Yue ^{[1
,2
]}

Huang, Yanni ^{[1
,2
]}

Long, Feiwu ^{[3
]}

Yang, Dongmei ^{[1
,2
]}

Huang, Yan ^{[1
,2
]}

Han, Yuanyuan ^{[1
,2
]}

Wu, Yanping ^{[3
,4
,5
]}

Zhong, Kai ^{[3
,4
,5
]}

Bu, Qian ^{[1
,2
,3
]}

Gao, Hong ^{[3
,4
,5
]}

Huang, Yina ^{[1
,2
,3
]}

机构：

[1] Sichuan Univ, West China Sch Publ Hlth, Chengdu 610041, Peoples R China

[2] Sichuan Univ, West China Hosp 4, Chengdu 610041, Peoples R China

[3] Sichuan Univ, Res Ctr Nutr Metab & Food Safety, West China PUMC CC Chen Inst Hlth, Chengdu 610041, Peoples R China

[4] Sichuan Univ, Coll Biomass Sci & Engn, Chengdu 610065, Peoples R China

[5] Sichuan Univ, Hlth Food Evaluat Res Ctr, Chengdu 610065, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 404卷

基金：

中国博士后科学基金;

关键词：

Fuzhuan brick tea; Theabrownin; Structural characterization; Hyperlipidemia; Intestinal lipid absorption; PU-ERH TEA; INTESTINAL-ABSORPTION; MICELLAR SOLUBILITY; CHOLESTEROL; POLYPHENOLS; HYPERLIPIDEMIA; INHIBITION; DIETARY;

D O I：

10.1016/j.foodchem.2022.134382

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Fuzhuan brick tea (FBT) is a post-fermented dark tea preferred by consumers for its excellent hypolipidemic activity, and theabrownin (TB) is the main bioactive composition in FBT. This work explored the structural and hypolipidemic properties of TB derived from Pingwu FBT, and investigated whether it exerted hypolipidemic activity by inhibiting intestinal lipid absorption. Structural characterization revealed that TB was an amorphous polymerized phenolic compound rich in hydroxyl and carboxyl groups with good thermostability. In vivo, TB and its fractions with different molecular weights (TB-LT3k, TB-3-10k, TB-10-30k, TB-30-100k, TB-GT100k) signif-icantly reduced the lipid levels of hyperlipidemia zebrafish (P < 0.05). Moreover, TB attenuated hyperlipidemia by inhibiting intestinal lipid absorption, as TB effectively bound to bile acids, inhibited enzymatic activity of pancreatic lipase and cholesterol esterase, influenced micelle formation, and decreased micellar cholesterol solubility. Results facilitated research on TB and offered support for its feasibility as a natural alternative to prevent hyperlipidemia.

引用

页数：11