Novel Nano-Sorbents Modified QuEChERS combined with GC-MS/MS for determination of pesticide residues used against Spodoptera littoralis in food stuff

The role of pesticides in the management of the destructive pest, Spodoptera littoralis is an essential element. Chlorpyrifos and Cypermethrin are the most commonly used insecticides in our country. Insect resistance against such insecticides enforced cultivars to increase the recommended doses which increases the pesticide residues in our food. This study's objective was to create a new methodology to measure the residue of Chlorpyrifos and Cypermethrin in potato and tomato yields. Chlorpyrifos showed high tolerance ratio equal to 380-fold in the field strain of S. littoralis compared with laboratory strain, while Cypermethrin showed a tolerance ratio of 5. 79-fold.A proposed modification of QuEChERS method to ensure the effectiveness of using different sorbent nanoparticles in the purification of both Chlorpyrifos and Cypermethrin residues from co-extracts can result in falsely favorable findings in order to guarantee the security of the commercially tomato and potato yields. A new, quick and easy analytical technique was created to determine Chlorpyrifos and Cypermethrin insecticide residues Using GC-MS/MS (gas chromatography-tandem mass spectrometry). In this method, Octadecyl-modified silica (C18), graphitized carbon black (GCB) and primary secondary amine (PSA) were converted to their corresponding nanoparticles, for the first time, and used in the sample purification step.Acetonitrile was used to extract the samples and measured using internal standards and matrix-matched calibration. With values of r2>0.99, all pesticides demonstrated good linearity in their respective ranges. With RSDs of 0.2 percent -14.4 percent, the average recoveries of the pesticide-spiked samples varied from 70.0 percent to -112.2 percent. The modified QuEChERS approach proved reliable and effective when used with tomato and potato samples to identify pesticide residues at trace levels.