The migration regularity and removal mechanism of antibiotic resistance genes during in situ enzymatic hydrolysis and anaerobic digestion of food waste

This study developed a high efficiency compound enzyme (fungal mash) produced in situ from food waste (FW) used for improving hydrolysis and anaerobic digestion (AD) efficiency of FW. Results showed that the soluble COD and methane yield were respectively increased by 67.80% and 16.58% after 24 h in situ enzymatic hy-drolysis of food waste by fungal mash. Furthermore, most of target ARGs in FW were also reduced by 45-94% after 24 h in situ enzymatic hydrolysis, while the total tested ARGs and intI1 were respectively further removed by 44-55% and 21-73% in subsequent AD process. In-depth analysis showed that fungal mash could effectively reduce potential hosts and control the horizontal transfer of ARGs during the in situ enzymatic hydrolysis and AD process. Ultimately, correlation analysis and redundancy analysis indicated that the evolution of bacterial communities and changes in intI1 where the common driving forces for the fate of ARGs.