Pollen concentration in the atmosphere is strongly affected by changes in meteorological factors, as well as urban and suburban vegetation in the study area. The identification of atmospheric pollen sources and estimates on their concentrations are critical for the quality of life for individuals affected by pollen allergy. In this study, we evaluated the effect of wind on the concentration levels of dominant pollen types (Cupressaceae/Taxaceae, Olea europaea, Pinaceae, Poaceae, Quercus spp., and Urticaceae) recorded in the atmosphere of Izmir, Turkey's 3rd largest city, in accordance with land cover over a 3-year period. Our results showed higher pollen concentrations for all taxa on days when wind direction was from the southeast toward the city. Elevations around the city, where arboreal taxa are widely distributed, particularly contributed to pollen concentrations through winds blowing from their directions. Besides these environmental elements, plants used in urban landscaping, such as Pinaceae, Olea europaea, and Cupressaceae/Taxaceae also contributed to pollen concentrations in the city's atmosphere. Although we observed no significant transport from the northern part of the city, we have determined that the agricultural lands in the western and southern parts contribute to the pollen concentrations of Poaceae and Olea europaea. Additionally, we recorded the highest pollen transport for all taxa when wind speed was within the range of 1.6-3.3 m/s, while pollen concentrations declined in higher wind speeds.