Gut microbiome-mediated glucose and lipid metabolism mechanism of star apple leaf polyphenol-enriched fraction on metabolic syndrome in diabetic mice

被引：1

作者：

Wang, Yajie ^{[1
]}

Jia, Yanan ^{[1
]}

Li, Shuqin ^{[1
]}

Li, Nannan ^{[1
]}

Zhou, Jingna ^{[1
]}

Liu, Junyu ^{[1
]}

Yang, Shuyu ^{[1
]}

Zhang, Min ^{[2
,3
]}

Panichayupakaranant, Pharkphoom ^{[4
]}

Chen, Haixia ^{[1
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effici, Tianjin 300072, Peoples R China

[2] Tianjin Agr Univ, Tianjin 300384, Peoples R China

[3] Tianjin Univ Sci & Technol, State Key Lab Nutr & Safety, Tianjin 300457, Peoples R China

[4] Prince Songkla Univ, Fac Pharmaceut Sci, Phytomedicine & Pharmaceut Biotechnol Excellence C, Hat Yai 90112, Thailand

来源：

PHYTOMEDICINE | 2023年 / 115卷

关键词：

Chrysophyllum cainito; Star apple leaf polyphenol; Metabolic syndrome; Gut microbiota; Diabetes; Glycolipid metabolism; INSULIN-RESISTANCE; IDENTIFICATION; MYRICETIN;

D O I：

10.1016/j.phymed.2023.154820

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: Diabetes is a kind of metabolic syndrome (MetS) that seriously threatens human health globally. The leaf of star apple (Chrysophyllum cainito L.) is an incompletely explored folk medicine on diabetes. And, the effects and mechanisms on diabetes complicated glycolipid metabolism disorders are unknown till now. Purpose: This study aimed to investigate the constituents of star apple leaf polyphenol enriched-fraction (SAP), and elucidate their treatment effects and mechanism on diabetes and accompanied other MetS. Methods: The components of SAP were tentatively identified by HPLC-Q-TOF-MS/MS. The antioxidant activity was determined by the scavenging of free radicals and hypoglycemic activities by inhibition of alpha-glucosidase in vitro. HepG2 cells were used for evaluating the alleviation effects of SAP on lipid accumulation. Streptozotocin and high-fat diet induced diabetic mice were grouped to evaluate the effects of different dosages of SAP. 16S rRNA was conducted to analysis gut microbiome-mediated glucose and lipid metabolism mechanism. Results: It showed that myricitrin was one of the main active constituents of SAP. SAP not only showed low IC50 on -glucosidase (24.427 +/- 0.626 mu g/mL), OH.(3.680 +/- 0.054 mu g/mL) and ABTS. (9.155 +/- 0.234 mu g/mL), but significantly induced the lipid accumulation in HepG2 cells (p < 0.05). SAP at 200 mg/kg.day significantly decreased the blood glucose, insulin and oral glucose tolerance test value (p < 0.05). The insulin resistance indexes and oxidative stress were alleviated after administration. SAP not only attenuated hepatic lipid deposition, but also reversed the hepatic glycogen storage. 16S rRNA sequencing results revealed that the interaction between SAP and gut microbiota led to the positive regulation of beneficial bacteria including Akkermansia, Unspecified S24_7, Alistipes and Unspecified_Ruminococcaceae, which might be one of the mechanisms of SAP on MetS. Conclusion: For the first time, this study explored the regulation effect of star apple leaf polyphenols on the hepatic glycolipid metabolism and studied the underlying mechanism from the view of gut microbiota. These findings indicated that SAP possesses great potential to serve as a complementary medicine for diabetes and associated MetS. It provided scientific evidence for folk complementary medicine on the treatment of diabetescomplicated multiple metabolic disorders.

引用

页数：12