Characterization of insoluble dietary fiber from Pleurotus eryngii and evaluation of Ch ra e i io of i l ble di y fib r f m Ple o ryngii d e al ti n of its effects on obesity-preventing or relieving effects via modulation of gut microbiota

The purpose of this study was to evaluate the obesity preventing or relieving effect of insoluble dietary fiber (IDF) fromPleurotus eryngii through its modulations on intestinal microbiota. The IDF was extracted The purpose of his study was to evaluate the obesity preventing or relieving eff ct of insolub e dietary fiber (IDF) fomPleurotus eryngii through its modultions on testina mirobiota. The IDF was extracte and structurally characterized through Fourier transforminfrared spectroscopy (FT-IR), X-ray and thermogravimetri (TG) analysis. The mice forin vivo experments were divided into obesity preventig and structurally c haracterized through Fourier transform infrared spectroscopy (FT-IR), X-ray and group thermogravmetrc(feeding high-fat(TG) diet analysiswhile Thegavage miceIDF forin vivosimultaneously) experimentsand obesitywere relevingdivided intogroup obesity(IDF interonpreventing aftergroup the (feding establishmenhigh-fatofdiet the whilobe se gavagmodel) IDFto evaluatesimultaneousl) its andhypolipidemc obesity effect.relieving The groupyield of(IDFP. eryngiiintervention IDF exracts was 56.49% (onP. eryngii dry wight base) with IDF content of 85.15% in the extracts. The in vivo after the establishment of the obes model) to evaluate its hypolipidemic effect The yield of P. eryngii IDF results showed that the body weight of the obesity-preventing groups was still increasing after intervention extracts was 56.49% (on P. eyngii dry weight base) with IDF content of 8 .15% in the exracts The in vivo with IDF, but the growth rate as lowered, and the abnormal lipid metabolim ad inflammaion syndromes results showed that the body weight of the obesity-preventing groups was still increasing after intervention ithwere IDF, alleviaed;but the the growthacetic rateacid was contentlowered of theand themedium abnormaand l owlip dos metabolismdietary f iberand finterventionlammation groupssyndrome was weresignif icantlyalleviated; higher the thanace icthat acidof the contentnormal of thegroup, mediumand the and lowstructure doseof th dietary intestinalfiber flora interventionwas impved.groups wasThe mice weight from the obesiy-reliving group decreased ignificantly. The abnormal lipid metablism and significantly higher than that of the normal group, and the structure of the intestinal flora was improved. The mice weight from the obesity-relieving group decreased sigifcantly. Th abnormal lipid metabolism and inflammation were alleviated. In conclusion, The IDF intervention decrease d the abnormal lipid metabolism iflammtion were alleviated. In conclusio, The IDF intervntion decrea ed the abnorma lip d meabolism and inflammation in both obesity-preventing and relieving experiments. The intestinal fl ora structure was and obviously inflamatinimproved. in P.botheryngiiIDF isobesity-preventing a good andcandidate reliev as ng hypoliideicexperimen s. agent.The