Enhanced mesophilic fermentation of waste activated sludge by integration with in-situ nitrate reduction

This study investigated the reduction of nitrate in a mesophilic waste activated sludge (WAS) fermentation system and determined the effect of nitrate reduction on the hydrolysis, acidogenesis and acetogenesis. Experimental results showed that the initial nitrate concentrations of 100, 200 and 400 mg/L were completely reduced in 1, 2 and 7 days, respectively. The destruction of volatile suspended solids was 1.2, 1.8 and 2.8 times, respectively, that without nitrate, demonstrating nitrate promoted the release of organic matter in sludge and enhanced the biodegradability of sludge organics. Moreover, batch tests using model substrates illustrated nitrate reduction promoted sludge hydrolysis and acetogenesis, but slightly inhibited acidogenesis. This study offers a feasible method to address two major problems currently faced by biological wastewater treatment plants, i.e. the overabundance of WAS and the lack of carbon sources for the denitrification process.