Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production

被引：2

作者：

Nam, Hyangsu ^{[1
]}

Kim, Taehyeon ^{[1
]}

Moon, Sunghyun ^{[1
]}

Ji, Yoonbin ^{[1
]}

Lee, Jong Bum ^{[1
]}

机构：

[1] Univ Seoul, Dept Chem Engn, 163 Seoulsiripdaero, Seoul 02504, South Korea

来源：

ISCIENCE | 2023年 / 26卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

DNA; EXPRESSION;

D O I：

10.1016/j.isci.2023.107089

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.

引用

页数：14