Presence of Aspergillus fumigatus with the TR34/L98H Cyp51A mutation and other azole-resistant aspergilli in the air of a zoological park

被引:0
|
作者
Alvarez-Perez, Sergio [1 ]
Garcia, Marta E. [1 ]
Martinez-Nevado, Eva [2 ]
Blanco, Jose L. [1 ]
机构
[1] Univ Complutense Madrid, Fac Vet Med, Dept Anim Hlth, Ave Puerta Hierro S-N, Madrid 28040, Spain
[2] ZOO AQUARIUM Madrid, Casa Campo s-n, Madrid 28011, Spain
关键词
Air sampling; Aspergillus; Azole resistance; cyp51A mutation; Zoological park; EPIDEMIOLOGIC CUTOFF VALUES; MIC DISTRIBUTIONS; ANTIFUNGAL SUSCEPTIBILITY; POLYPHASIC TAXONOMY; CLINICAL-SAMPLES; FUNGAL LOADS; WILD; SPP; IDENTIFICATION; ENVIRONMENTS;
D O I
10.1016/j.rvsc.2023.104993
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Antifungal-resistant fungi, including Aspergillus fumigatus and other Aspergillus species, pose an urgent threat to human and animal health. Furthermore, the environmental route of azole resistance selection due to the widespread use of azole fungicides in crop protection and other applications is a major public health issue. Although environmental surveillance of fungi is frequently performed in many zoological parks and wildlife rehabilitation centers, the antifungal susceptibility of recovered isolates is only rarely analyzed, which precludes a clear assessment of the threat posed by these fungi to captive animals. In this study, we assessed the presence of airborne azole-resistant Aspergillus spp., including the so-called 'cryptic species' (i.e., species which are pheno-typically similar to more well-known aspergilli but clearly constitute different phylogenetic lineages) in a zoological park located in the city of Madrid, Spain. In general, our results revealed a low prevalence A. fumigatus and cryptic aspergilli with decreased susceptibility to azoles. However, we detected an A. fumigatus isolate with the TR34/L98H mutation in the gene encoding the lanosterol14 alpha-demethylase (Cyp51A), consisting of a tandem repeat of 34 base pairs in the promoter region and a lysine to histidine substitution at codon 98. Notably, this TR34/L98H mutation has been linked to the environmental route of azole resistance selection, thus highlighting the 'One Health' dimension of the emerging problem of antifungal resistance. In this context, continuous envi-ronmental surveillance of azole-resistant aspergilli in zoological parks and other similar animal facilities is recommended.
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页数:8
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