Antioxidant, toxic and antimicrobial activity of Rosmarinus officinalis, Ruta graveolens and Juglans regia against Helicobacter pylori

Helicobacter pylori is a Gram negative bacteria with a high prevalence worldwide, causing gastric and duodenal ul-cers and cancer, it is one of the main public health problems in Mexico. This work evaluates the in vitro antioxidant, toxic, antibacterial activity, and the ability to inhibit the biofilm formed by H. pylori, of the methanolic extract Juglans regia (EMJR) and crude ethanolic extracts of the species Rosma-rinus officinalis (EERO) and Ruta graveolens (EERG) collected in the state of Nuevo Leon, Mexico. Through phytochemical screening, the presence of secondary metabolites was qua-litatively determined. The antioxidant capacity by the DPPH method showed that EMJR was the most active with an IC50 of 2.759 pg/mL. The EERG and EERO developed inhibition halos of 11 and 16 mm and a MIC of 0.136 and 0.51 mg/ mL respectively, only the EERO inhibits biofilm formation by 83.7%. Toxicity tests on Artemia salina showed weak to moderate toxicity. The results show the potential use of the studied extracts as alternative sources in the search for new treatments against H. pylori.