Bioinspired nanotopography on 3D printed tissue scaffold to impart mechanobactericidal and osteogenic activities

The great demand for bone grafts has motivated the development of tissue scaffolds with osteogenic activity, whereas the risk of implant-associated infection, especially given the rise of antimicrobial resistance, has compelled the development of scaffolds with innovative antimicrobial strategies. Bioinspired mechanobactericidal nanostructures are highly appealing as an alternative to traditional chemical approaches. This study presents an innovative spin-coating setup for the generation of nanotopography on the surfaces of a threedimensional (3D)-printed porous polylactide (PLA) scaffold based on the principle of polymer demixing. The nanostructured PLA surface exhibited excellent bactericidal activity by contact killing of P. aeruginosa (86.60 % dead cells in 24 h) and S. aureus (92.36 %). The nanoscale topography supported the attachment and proliferation of pre-osteoblasts and better supported osteogenic differentiation than the unmodified scaffold. These findings demonstrate a single-step spin coating to yield nanotopography on 3D-printed polymer scaffolds that concurrently impart mechanobactericidal and osteogenic activities. Taken together, this work has important implications for engineering the next-generation 3D printed bioactive tissue scaffolds.