Panoramic quantitative phase imaging of adherent live cells in a microfluidic environment

被引:0
|
作者
Ma, Ying [1 ,2 ,3 ]
Dai, Taiqiang [4 ,5 ,6 ]
Lei, Yunze [1 ,2 ,3 ]
Zhang, Linlin [4 ,5 ,6 ]
Ma, Lin [1 ,2 ,3 ]
Liu, Min [1 ,2 ,3 ]
An, Sha [1 ,2 ,3 ]
Zheng, Juanjuan [1 ,2 ,3 ]
Zhuo, Kequn [1 ,2 ,3 ]
Kong, Liang [4 ,5 ,6 ]
Gao, Peng [1 ,2 ,3 ]
机构
[1] Xidian Univ, Sch Phys, Xian 710071, Peoples R China
[2] Minist Educ, Key Lab Optoelect Percept Complex Environm, Xian, Peoples R China
[3] Univ Shaanxi Prov, Engn Res Ctr Funct Nanomat, Xian, Peoples R China
[4] Fourth Mil Med Univ, State Key Lab Mil Stomatol, Xian 710000, Peoples R China
[5] Fourth Mil Med Univ, Natl Clin Res Ctr Oral Dis, Xian 710000, Peoples R China
[6] Fourth Mil Med Univ, Shaanxi Engn Res Ctr Dent Mat & Adv Manufacture, Sch Stomatol, Xian 710000, Peoples R China
基金
中国国家自然科学基金;
关键词
CORRELATION SPECTROSCOPY; SINGLE-CELL; TECHNOLOGIES; MICROSCOPY;
D O I
10.1364/BOE.498602
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Understanding how cells respond to external stimuli is crucial. However, there are a lack of inspection systems capable of simultaneously stimulating and imaging cells, especially in their natural states. This study presents a novel microfluidic stimulation and observation system equipped with flat-fielding quantitative phase contrast microscopy (FF-QPCM). This system allowed us to track the behavior of organelles in live cells experiencing controlled microfluidic stimulation. Using this innovative imaging platform, we successfully quantified the cellular response to shear stress including directional cellular shrinkage and mitochondrial distribution change in a label-free manner. Additionally, we detected and characterized the cellular response, particularly mitochondrial behavior, under varying fluidic conditions such as temperature and drug induction time. The proposed imaging platform is highly suitable for various microfluidic applications at the organelle level. We advocate that this platform will significantly facilitate life science research in microfluidic environments.
引用
收藏
页码:5182 / 5198
页数:17
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