Comparative Physiological and Transcriptome Analyses of Tolerant and Susceptible Cultivars Reveal the Molecular Mechanism of Cold Tolerance in Anthurium andraeanum

被引:5
|
作者
Dou, Na [1 ]
Li, Li [1 ]
Fang, Yifu [2 ]
Fan, Shoujin [1 ]
Wu, Chunxia [1 ]
机构
[1] Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Plant Stress, 88 Wenhua East Rd, Jinan 250014, Peoples R China
[2] Shandong Prov Acad Forestry, Inst Ornamental Plants, Wenhua East Rd 42, Jinan 250010, Peoples R China
关键词
Anthurium andraeanum; cold stress; transcriptome; WGCNA; plant hormone signal; trehalose; ribosomal protein; HEAT-SHOCK-PROTEIN; ARABIDOPSIS-THALIANA; RIBOSOMAL-PROTEIN; STRESS TOLERANCE; FREEZING TOLERANCE; PLANT DEVELOPMENT; GENE; EXPRESSION; OVEREXPRESSION; METABOLISM;
D O I
10.3390/ijms25010250
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Anthurium andraeanum is a tropical ornamental flower. The cost of Anthurium production is higher under low temperature (non-freezing) conditions; therefore, it is important to increase its cold tolerance. However, the molecular mechanisms underlying the response of Anthurium to cold stress remain elusive. In this study, comparative physiological and transcriptome sequencing analyses of two cultivars with contrasting cold tolerances were conducted to evaluate the cold stress response at the flowering stage. The activities of superoxide dismutase and peroxidase and the contents of proline, soluble sugar, and malondialdehyde increased under cold stress in the leaves of the cold tolerant cultivar Elegang (E) and cold susceptible cultivar Menghuang (MH), while the soluble protein content decreased in MH and increased in E. Using RNA sequencing, 24,695 differentially expressed genes (DEGs) were identified from comparisons between cultivars under the same conditions or between the treatment and control groups of a single cultivar, 9132 of which were common cold-responsive DEGs. Heat-shock proteins and pectinesterases were upregulated in E and downregulated in MH, indicating that these proteins are essential for Anthurium cold tolerance. Furthermore, four modules related to cold treatment were obtained by weighted gene co-expression network analysis. The expression of the top 20 hub genes in these modules was induced by cold stress in E or MH, suggesting they might be crucial contributors to cold tolerance. DEGs were significantly enriched in plant hormone signal transduction pathways, trehalose metabolism, and ribosomal proteins, suggesting these processes play important roles in Anthurium's cold stress response. This study provides a basis for elucidating the mechanism of cold tolerance in A. andraeanum and potential targets for molecular breeding.
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页数:28
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