Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts

被引：7

作者：

Le, Violeta ^{[1
]}

Sukhikh, Andrey ^{[2
]}

Larichev, Timothy ^{[3
]}

Ivanova, Svetlana ^{[1
,4
]}

Prosekov, Alexander ^{[5
]}

Dmitrieva, Anastasia ^{[1
]}

机构：

[1] Kemerovo State Univ, Nat Nutraceut Biotesting Lab, Kemerovo 650043, Russia

[2] Kemerovo State Univ, Lab Physicochem Studies Pharmacol Act & Nat Cpds, Kemerovo 650043, Russia

[3] Kemerovo State Univ, Dept Fundamental & Appl Chem, Kemerovo 650043, Russia

[4] Kemerovo State Univ, Dept Gen Math & Informat, Kemerovo 650043, Russia

[5] Kemerovo State Univ, Lab Biocatalysis, Kemerovo 650043, Russia

来源：

MOLECULES | 2023年 / 28卷 / 04期

关键词：

Ginkgo biloba; callus cultures; biologically active substances; highly effective chromatography; antioxidant activity; geroprotective properties; DEEP EUTECTIC SOLVENTS; FLAVONOID EXTRACTION; EFFICIENT EXTRACTION; LEAVES; SEPARATION; CHROMATOGRAPHY; PURIFICATION; AGLYCONES; LEAF;

D O I：

10.3390/molecules28041560

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The work reveals the results of studying the content of biologically active substances in samples of extracts of Ginkgo biloba callus cultures. Callus cultures grown in vitro on liquid nutrient media were the objects of the study. Considering various factors affecting the yield of the target components during extraction, the volume fraction of the organic modifier in the extracting mixture, the temperature factor, and the exposure time were identified as the main ones. The maximum yield of extractive substances (target biologically active substances with a degree of extraction of at least 50%) from the samples of callus culture extracts was detected at a ratio of extragent of 70% ethanol, a temperature of 50 degrees C, and exposure time of 6 h. Flavonoids, such as luteolin, quercetin, isoramentin, kaempferol, and amentoflavone, were isolated in the extract samples. As a result of column chromatography, fractions of individual biologically active substances (bilobalide, ginkgolide A, B, and C) were determined. The proposed schemes are focused on preserving the nativity while ensuring maximum purification from associated (ballast) components. Sorbents (Sephadex LH-20, poly-amide, silica gel) were used in successive stages of chromatography with rechromatography. The degree of purity of individually isolated substances was at least 95%.

引用

页数：19