Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications

被引：23
|
作者：
Vrablova, Veronika [1 ]
Kosutova, Natalia
Blsakova, Anna [1 ]
Bertokova, Aniko [2 ]
Kasak, Peter [3 ]
Bertok, Tomas [1 ,2 ]
Tkac, Jan [1 ,2 ]
机构：
[1] Slovak Acad Sci, Inst Chem, Dubravska Cesta 9, Bratislava 84538, Slovakia
[2] Glycanost Sro, Kudlakova 7, Bratislava 84101, Slovakia
[3] Qatar Univ, Ctr Adv Mat, POB 2713, Doha, Qatar
来源：
BIOTECHNOLOGY ADVANCES | 2023年 / 67卷
关键词：
Extracellular vesicles; Exosomes; Diagnostics; Biomolecular corona; Glycans; Lectins; PROTEINS; EXOSOMES; CALCIFICATION; RESISTANCE; GLYCANS; PLASMA;
D O I：
10.1016/j.biotechadv.2023.108196
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This review provides a comprehensive overview of our understanding of the role that glycans play in the for-mation, loading and release of extracellular vesicles (EVs). The capture of EVs (typically with a size of 100-200 nm) is described, including approaches based on glycan recognition with glycan-based analysis offering highly sensitive detection of EVs. Furthermore, detailed information is provided about the use of EV glycans and glycan processing enzymes as potential biomarkers, therapeutic targets or tools applied for regenerative medicine. The review also provides a short introduction into advanced methods for the characterization of EVs, new insights into the biomolecular corona covering EVs and bioanalytical tools available for glycan analysis.
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收藏
页数：23
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