Eight Holstein cows were used in a replicated 4 x 4 Latin square design study to determine the effects of partially replacing canolameal (CM) with 5%, 7.5%, and 10% camelina expellermeal (CEM) on production and milk fatty acid profiles. Replacing CM with CEM did not affect feed intake and milk yield, but milk contents of fat and protein decreased linearly. Feeding increasing amounts of CEM linearly increased milk contents of C18:2n6, C18:3n3, cis-9, trans-11 CLA, trans-10, cis-12 CLA, and total CLA. CEM can be fed up to 10% of dietary DM without negatively affecting production and can increase milk contents of omega-3 fatty acids.