Dual-Labeled Octahedral Gold and Magnetic Microsphere Electrochemical Immunosensor for Ultrasensitive Determination of Carbohydrate Antigen-199 (CA199) by Differential Pulse Voltammetry (DPV)

被引：1

作者：

Wang, Hongdan ^{[1
,2
]}

Yuan, Wei ^{[1
]}

Zhang, Liqiu ^{[1
]}

Xie, Baogang ^{[3
]}

Cheng, Qiong ^{[1
]}

Zhang, Ningwen ^{[1
]}

机构：

[1] Jiaxing Univ, Coll Biol, Jiaxing Key Lab Mol Recognit & Sensing, Chem Sci & Engn, Jiaxing, Peoples R China

[2] Jiaxing Nanhu Univ, Coll Adv Mat Engn, Jiaxing, Zhejiang, Peoples R China

[3] Jiaxing Univ, Dept Pharmaceut, Med Coll, Jiaxing, Peoples R China

来源：

ANALYTICAL LETTERS | 2024年 / 57卷 / 05期

关键词：

Aminated magnetic nanoparticles; differential pulse voltammetry (DPV); dual enzyme catalysis; carbohydrate antigen-199 (CA199); octahedral gold; NANOPARTICLES;

D O I：

10.1080/00032719.2023.2227907

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

To precisely detect CA199 antigen, a novel electrochemical immunosensor based on horseradish peroxidase (HRP) and glucose oxidase (GOD) double enzyme system was prepared. Thiol was adsorbed on the surface of nano-octahedral gold through Au-S self-assembly; the second antibody was labeled by glutaraldehyde ligase as a signal amplification reagent. Amino magnetic microspheres were used to connect GOD and bovine serum albumin (BSA) through glutaraldehyde. The biosensor displayed low detection limits of 0.2 U/mL and wide linear range (0.5-200 U/mL). This novel immunosensor improves the sensitivity of current detection, showing promising application prospects in the clinical immunoassay detection of CA199.

引用

页码：841 / 854

页数：14