A Chitosan-Based Biomaterial Combined with Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Skin Regeneration

被引:1
|
作者
Humenik, Filip [1 ]
Danko, Jan [1 ,2 ]
Kresakova, Lenka [1 ]
Vdoviakova, Katarina [1 ]
Vrabec, Vladimir [3 ]
Vasilova, Emilia [3 ]
Giretova, Maria [4 ]
Toth, Stefan [5 ]
Fagova, Zuzana [5 ]
Babik, Jan [6 ]
Medvecky, Lubomir [1 ,4 ]
机构
[1] Univ Vet Med & Pharm Kosice, Dept Morphol Sci, Kosice 04181, Slovakia
[2] Educ Sci & Res Inst AGEL, Bratislava 81106, Slovakia
[3] Univ Vet Med & Pharm Kosice, Clin Birds Exot & Free Living Anim, Kosice 04181, Slovakia
[4] Inst Mat Res SAS, Div Funct & Hybrid Syst, Kosice 04001, Slovakia
[5] Univ Pavol Jozef Safarik, Dept Histol & Embryol, Kosice 04180, Slovakia
[6] AGEL Hosp, Clin Burns & Reconstruct Med, SK-04015 Kosice, Slovakia
关键词
biomaterial; wound healing; mesenchymal stem cell; conditioned medium; ENDOTHELIAL GROWTH-FACTOR; ANGIOGENESIS; MIGRATION; HYDROGEL; FRACTION;
D O I
10.3390/ijms242216080
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this study was to provide a beneficial treatment effect of novel chitosan bio-polymeric material enriched with mesenchymal stem cell products derived from the canine adipose tissue (AT-MSC) on the artificial skin defect in a rabbit model. For the objectivity of the regeneration evaluation, we used histological analysis and a scoring system created by us, taking into account all the attributes of regeneration, such as inflammatory reaction, necrosis, granulation, formation of individual skin layers and hair follicles. We observed an acceleration and improvement in the healing of an artificially created skin defect after eight and ten weeks in comparison with negative control (spontaneous healing without biomaterial). Moreover, we were able to described hair follicles and epidermis layer in histological skin samples treated with a chitosan-based biomaterial on the eighth week after grafting.
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页数:14
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