Full-scale upgrade activated sludge to continuous-flow aerobic granular sludge: Implementing microaerobic-aerobic configuration with internal separators

Aerobic granular sludge (AGS) has been successfully used in sequencing batch reactors. However, their application to existing continuous-flow systems remains challenging. In this study, a novel microaerobic-aerobic configuration with internal separators was implemented in a full-scale municipal wastewater treatment facility with a nominal capacity of 2.5 x 104 m3 d-1. Sludge characteristics, pollutant removal and associated pathways, shifts in the microbial community, and underlying granulation mechanisms were investigated. Following a two-month operation period, the transition from flocculent-activated sludge to well-defined AGS with distinct boundaries and compact structures was successfully achieved. The average size of sludge increased from 31.9 to 138.5 mu m, with granules larger than 200 mu m constituting 28.9 % of the total sludge and SVI30 averaging 51.4 +/- 8.2 mL g-1. The 95th percentile effluent COD, NH4+-N, and TN concentrations were 35.0, 1.2, and 13.3 mg L-1, respectively. The primary pathways for pollutant removal were identified as simultaneous nitrification, denitrification, and phosphorus removal within the microaerobic tanks. The enrichment of denitrifying phosphorus-accumulating organisms, including Hydrogenophaga, Accumulibacter, Azospira, Dechloromonas, and Pseudomonas, provides an essential microbial foundation. Furthermore, computational fluid dynamics modeling revealed that the incorporation of internal separators in aerobic tanks induced shifts in the flow pattern, transitioning from a single-circulation cell to multiple vortical cells. This alteration amplified the local velocity gradients, generating the required shear forces to drive granulation. Moreover, mass balance analysis revealed that the microaerobic and aerobic tanks operated under feast and famine conditions, respectively, creating a microbial selection pressure that favored granulation. This process eliminates the need for external clarifiers, resulting in a footprint reduction of 38.2 % and one-third energy savings for sludge reflux. This study offers valuable insights into the application of continuous-flow AGS to upgrade existing activated sludge systems with limited retrofitting requirements.