Structure of the Mating-Type Genes and Mating Systems of Verpa bohemica and Verpa conica (Ascomycota, Pezizomycotina)

被引:1
|
作者
Sun, Wenhua [1 ]
Liu, Wei [2 ]
Cai, Yingli [3 ]
Shi, Xiaofei [2 ]
Wu, Liyuan [2 ]
Zhang, Jin [2 ,4 ]
Er, Lingfang [2 ,4 ]
Huang, Qiuchen [2 ,5 ]
Yin, Qi [1 ]
Zhao, Zhiqiang [6 ]
He, Peixin [1 ]
Yu, Fuqiang [2 ]
机构
[1] Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou 450002, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
[3] Yunnan Acad Agr Sci, Inst Agroprod Proc, Kunming 650221, Peoples R China
[4] Yunnan Agr Univ, Coll Resource & Environm, Kunming 650100, Peoples R China
[5] Southwest Forestry Univ, Sch Biodivers Conservat, Kunming 650224, Peoples R China
[6] Agr Technol Promot Stn Zhuoni Cty, Gannan 747600, Peoples R China
关键词
fungi; life cycle; homothallism; heterothallism; MAT1-1-1; MAT1-2-1; MAT1-1-10; MAT1-1-11; FRUITING BODIES; EVOLUTION; IDENTIFICATION; MORCHELLACEAE; NOMENCLATURE; SUPPORTS; LOCUS; SEX;
D O I
10.3390/jof9121202
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Verpa spp. are potentially important economic fungi within Morchellaceae. However, fundamental research on their mating systems, the key aspects of their life cycle, remains scarce. Fungal sexual reproduction is chiefly governed by mating-type genes, where the configuration of these genes plays a pivotal role in facilitating the reproductive process. For this study, de novo assembly methodologies based on genomic data from Verpa spp. were employed to extract precise information on the mating-type genes, which were then precisely identified in silico and by amplifying their single-ascospore populations using MAT-specific primers. The results suggest that the MAT loci of the three tested strains of V. bohemica encompassed both the MAT1-1-1 and MAT1-2-1 genes, implying homothallism. On the other hand, amongst the three V. conica isolates, only the MAT1-1-1 or MAT1-2-1 genes were present in their MAT loci, suggesting that V. conica is heterothallic. Moreover, bioinformatic analysis reveals that the three tested V. bohemica strains and one V. conica No. 21110 strain include a MAT1-1-10 gene in their MAT loci, while the other two V. conica strains contained MAT1-1-11, exhibiting high amino acid identities with those from corresponding Morchella species. In addition, MEME analysis shows that a total of 17 conserved protein motifs are present among the MAT1-1-10 encoded protein, while the MAT1-1-11 protein contained 10. Finally, the mating type genes were successfully amplified in corresponding single-ascospore populations of V. bohemica and V. conica, further confirming their life-cycle type. This is the first report on the mating-type genes and mating systems of Verpa spp., and the presented results are expected to benefit further exploitation of these potentially important economic fungi.
引用
收藏
页数:16
相关论文
共 50 条
  • [1] Which MAT gene? Pezizomycotina (Ascomycota) mating-type gene nomenclature reconsidered
    Wilken, P. Markus
    Steenkamp, Emma T.
    Wingfield, Michael J.
    De Beer, Z. Wilhelm
    Wingfield, Brenda D.
    [J]. FUNGAL BIOLOGY REVIEWS, 2017, 31 (04) : 199 - 211
  • [2] TRANSPOSABLE MATING-TYPE GENES IN YEASTS
    LEUPOLD, U
    [J]. NATURE, 1980, 283 (5750) : 811 - 812
  • [3] COMBINATORICS AND VARIETY OF MATING-TYPE SYSTEMS
    BULL, JJ
    PEASE, CM
    [J]. EVOLUTION, 1989, 43 (03) : 667 - 671
  • [4] IRREGULAR TRANSPOSITIONS OF MATING-TYPE GENES IN YEAST
    KLAR, AJS
    HICKS, JB
    STRATHERN, JN
    [J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1980, 45 : 983 - 990
  • [5] STRUCTURE AND FUNCTION OF MATING-TYPE GENES IN COCHLIOBOLUS SPP AND ASEXUAL FUNGI
    TURGEON, BG
    SHARON, A
    WIRSEL, S
    YAMAGUCHI, K
    CHRISTIANSEN, SK
    YODER, OC
    [J]. CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1995, 73 : S778 - S783
  • [6] MATING-TYPE GENES AND THE CONTROL OF EXPRESSION OF FRUITING GENES IN BASIDIOMYCETES
    WESSELS, JGH
    [J]. ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY, 1987, 53 (05): : 307 - 316
  • [7] Mating-Type Genes and MAT Switching in Saccharomyces cerevisiae
    Haber, James E.
    [J]. GENETICS, 2012, 191 (01) : 33 - 64
  • [8] Mating-type genes for classical strain improvements of ascomycetes
    Pöggeler, S
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 56 (5-6) : 589 - 601
  • [9] Mating-type genes and hyphal fusions in filamentous basidiomycetes
    Raudaskoski, Marjatta
    [J]. FUNGAL BIOLOGY REVIEWS, 2015, 29 (3-4) : 179 - 193
  • [10] TRANSPOSITION OF A TANDEM DUPLICATION OF YEAST MATING-TYPE GENES
    HABER, JE
    ROGERS, DT
    [J]. NATURE, 1982, 296 (5859) : 768 - 770