Comparative analysis of the physicochemical properties and biological activities of Ziziphus Jujuba cv. Goutouzao polysaccharides obtained by fractional precipitation

Polysaccharides are the main active constituents of Jujuba, yet there are few researches on Ziziphus Jujuba cv. Goutouzao polysaccharides. Traditional separation and purification method of Jujuba polysaccharides was ion-exchange column chromatography, which is complicated and time-consuming. In this study, four polysaccharide components (SJP-1, SJP-2, SJP-3, and SJP-4) were obtained from crude Ziziphus Jujuba cv. Goutouzao polysaccharides (SJP) by ethanol fractional precipitation which are higher convenience than traditional method. The physicochemical properties, immune activities, and antioxidant activities (free radical scavenging ability, reducing power, lipid peroxidation, metal chelating power, inhibition of protein oxidative damage, protective effects of HL-7702 cell damage) of SJP and SJP-1-SJP-4 were investigated to determine its major active constituent. These results showed that SJP-4 had the highest antioxidant and immune activities, which could be related to its monosaccharide composition (Ara, 29.5%; Man, 10.0%; Glc, 16.9%; and Gal, 28.2%) and low molecular weight (0.73 x 10(4) Da). These results provide a scientific basis for the comprehensive development and utilization of Ziziphus Jujuba cv. Goutouzao in the food, medicine, and health product industry.