Genomic Insights and Synthetic Biology Applications of Marine Actinomycete Streptomyces griseoincarnatus HNS054

被引：0
|
作者：
Wang, Qinghua [1 ,2 ]
Zhao, Jing [1 ,2 ,3 ]
Liu, Zhaoyuan [1 ,2 ]
Ding, Shaoxiong [1 ,2 ,3 ]
Huang, Zhiyong [4 ]
Chen, Jun [1 ,2 ,3 ]
机构：
[1] Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China
[2] Xiamen Univ, Coll Ocean & Earth Sci, Dept Marine Biol Sci & Technol, Xiamen 361102, Peoples R China
[3] Xiamen Univ, State Prov Joint Engn Lab Marine Bioprod & Technol, Xiamen 361102, Peoples R China
[4] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 06期
基金：
中国国家自然科学基金;
关键词：
marine actinomycete; chassis; natural product; biosynthetic gene cluster; rapid growth; MSGE; HETEROLOGOUS EXPRESSION; ESCHERICHIA-COLI; COELICOLOR; GROWTH; GENES; CELL;
D O I：
10.3390/ijms25063127
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The marine bacterium Streptomyces sp. HNS054 shows promise as a platform for producing natural products. Isolated from a marine sponge, HNS054 possesses several desirable traits for bioengineering: rapid growth, salt tolerance, and compatibility with genetic tools. Its genome contains 21 potential biosynthetic gene clusters, offering a rich source of natural products. We successfully engineered HNS054 to increase the production of aborycin and actinorhodin by 4.5-fold and 1.2-fold, respectively, compared to S. coelicolor M1346 counterparts. With its unique features and amenability to genetic manipulation, HNS054 emerges as a promising candidate for developing novel marine-derived drugs and other valuable compounds.
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页数：14
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