Heterologous Production of the C33-C45 Polyketide Fragment of Anticancer Apratoxins in a Cyanobacterial Host

被引：2

作者：

Dhakal, Dipesh ^{[1
,2
]}

Kallifidas, Dimitris ^{[1
,2
]}

Chen, Manyun ^{[1
,2
]}

Kokkaliari, Sofia ^{[1
,2
]}

Chen, Qi-Yin ^{[1
,2
]}

Paul, Valerie J. ^{[3
]}

Ding, Yousong ^{[1
,2
]}

Luesch, Hendrik ^{[1
,2
]}

机构：

[1] Univ Florida, Dept Med Chem, Gainesville, FL 32610 USA

[2] Univ Florida, Ctr Nat Prod Drug Discovery & Dev CNPD3, Gainesville, FL 32610 USA

[3] Smithsonian Marine Stn, Ft Pierce, FL 34949 USA

来源：

ORGANIC LETTERS | 2023年 / 25卷 / 13期

基金：

美国国家卫生研究院;

关键词：

BIOLOGICAL EVALUATION; EXPRESSION; POTENT;

D O I：

10.1021/acs.orglett.3c00462

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A polyketide synthase subcluster of cytotoxic apratoxin A was isolated from a Moorena bouillonii environmental DNA library and engineered with a thioesterase II domain for heterologous expression in the filamentous cyanobacterium Anabaena sp. PCC7120. Further engineering with a rhamnose-inducible promoter led to the production of (2R,3R,5R,7R)-3,7-dihydroxy-2,5,8,8-tetrame-thylnonanoic acid, a stereogenically rich chiral building block that is important to the efficient synthesis of apratoxin analogues, representing the first synthetic biology attempt for this type of polyketide fragment.

引用

页码：2238 / 2242

页数：5

共 3 条

[1] Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-α subunit in a heterologous host
Tooley, AJ
Cai, YPA
Glazer, AN
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (19) : 10560 - 10565
[2] Evaluation of Streptomyces coelicolor A3(2) as a heterologous expression host for the cyanobacterial protein kinase C activator lyngbyatoxin A
Jones, Adam C.
Ottilie, Sabine
Eustaquio, Alessandra S.
Edwards, Daniel J.
Gerwick, Lena
Moore, Bradley S.
Gerwick, William H.
FEBS JOURNAL, 2012, 279 (07) : 1243 - 1251
[3] Heterologous production of five Hepatitis C virus-derived antigens in three Saccharomyces cerevisiae host strains
Parolin, C
Dal Corso, A
Alberghina, L
Porro, D
Branduardi, P
JOURNAL OF BIOTECHNOLOGY, 2005, 120 (01) : 46 - 58

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