Untargeted lipidomics approach using LC-Orbitrap HRMS to discriminate lard from beef tallow and chicken fat for the authentification of halal

被引:0
|
作者
Windarsih, A. [1 ,2 ]
Bakar, N. K. A. [1 ]
Rohman, A. [3 ,4 ]
Riswanto, F. D. O. [5 ]
Erwantof, Y. [6 ]
机构
[1] Univ Malaya, Dept Chem, Fac Sci, Kuala Lumpur 50603, Malaysia
[2] Natl Res & Innovat Agcy BRIN, Res Ctr Food Technol & Proc PRTPP, Yogyakarta 55861, Indonesia
[3] Univ Gadjah Mada, Dept Pharmaceut Chem, Fac Pharm, Yogyakarta 55281, Indonesia
[4] Univ Gadjah Mada, Inst Halal Ind & Syst PUI PT IHIS, Ctr Excellence, Yogyakarta 55281, Indonesia
[5] Univ Sanata Dharma, Fac Pharm, Div Pharmaceut Anal & Med Chem, Campus III Paingan, Yogyakarta 55282, Indonesia
[6] Univ Gadjah Mada, Fac Anim Sci, Yogyakarta 55281, Indonesia
关键词
Chemometrics; Halal Authentication; Lard; LC-HRMS; Metabolomics; Untargeted; MASS SPECTROMETRY; AUTHENTICATION; QUALITY; CHEMOMETRICS; SPECTROSCOPY; FOODOMICS; SAFETY; MS;
D O I
10.3989/gya.0980221
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This research aimed to perform a lipidomics study using liquid chromatography-high resolution mass spectrometry (LCHRMS) to identify lard, beef tallow and chicken fat. A total of 292, 345, and 403 lipid compounds were observed in lard, beef tallow, and chicken fat, respectively. The lipid groups of AcHexStE (acyl hexosyl stigmasterol ester), biotinylPE (biotinylphosphoetanolamine), LPC (lysophosphatidylcholine), MePC (monoetherphosphatidylcholine), PC (phosphatidylcholine) and PI (phosphoinocitol) were found to be specific for lard. The principal component analysis ( PCA) and partial least square-discriminant analysis (PLS-DA) successfully differentiated lard from beef tallow and chicken fat. This research suggested that the untargeted lipidomics technique using LC-HRMS combined with chemometrics could be used to discriminate lard from beef tallow and chicken fat. This method is a promising technique for the detection of lard adulteration in beef tallow and chicken fat for halal authentication purposes.
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页数:12
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