Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations

被引：1

作者：

Krason, Marcin Z. ^{[1
,2
,3
]}

Paradowska, Anna ^{[1
]}

Fronczek, Martyna ^{[1
,4
]}

Lejawa, Mateusz ^{[1
,4
]}

Kamienska, Natalia ^{[3
]}

Krejca, Michal ^{[2
]}

Kolanowska, Anna ^{[5
]}

Boncel, Slawomir ^{[5
,6
]}

Radomski, Marek W. ^{[7
]}

机构：

[1] Silesian Pk Med Technol Kardiomed Silesia, PL-41800 Zabrze, Poland

[2] Med Univ Lodz, Cardiac Surg Dept, PL-90419 Lodz, Poland

[3] Med Univ Silesia, Silesian Ctr Heart Dis Zabrze, Dept Cardiac Vasc & Endovasc Surg & Transplantol, PL-41800 Zabrze, Poland

[4] Med Univ Silesia, Fac Med Sci Zabrze, Dept Pharmacol, PL-40055 Katowice, Poland

[5] Silesian Tech Univ, Fac Chem, Dept Organ Chem Bioorgan Chem & Biotechnol, PL-44100 Gliwice, Poland

[6] Silesian Tech Univ, Ctr Organ & Nanohybrid Elect, PL-44100 Gliwice, Poland

[7] Univ Saskatchewan, Coll Med, Dept Anat Physiol & Pharmacol, Saskatoon, SK S7N 5E5, Canada

来源：

PHARMACEUTICS | 2023年 / 15卷 / 10期

关键词：

graphene oxide; aqueous dispersion; stability of dispersion; nanoparticle size; ion-induced aggregation; bovine serum albumin; AQUEOUS-SOLUTIONS; OXIDATIVE STRESS; SIZE; CYTOTOXICITY; CHEMISTRY; FUNCTIONALIZATION; NANOMATERIALS; HYDROLYSIS; ADSORPTION; VALUES;

D O I：

10.3390/pharmaceutics15102495

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Graphene oxide's (GO) intravascular applications and biocompatibility are not fully explored yet, although it has been proposed as an anticancer drug transporter, antibacterial factor or component of wearable devices. Bivalent cations and the number of particles' atom layers, as well as their structural oxygen content and pH of the dispersion, all affect the GO size, shape, dispersibility and biological effects. Bovine serum albumin (BSA), an important blood plasma protein, is expected to improve GO dispersion stability in physiological concentrations of the precipitating calcium and magnesium cations to enable effective and safe tissue perfusion. Methods: Four types of GO commercially available aqueous dispersions (with different particle structures) were diluted, sonicated and studied in the presence of BSA and physiological cation concentrations. Nanoparticle populations sizes, electrical conductivity, zeta potential (Zetasizer NanoZS), structure (TEM and CryoTEM), functional groups content (micro titration) and dispersion pH were analyzed in consecutive preparation stages. Results: BSA effectively prevented the aggregation of GO in precipitating concentrations of physiological bivalent cations. The final polydispersity indexes were reduced from 0.66-0.91 to 0.36-0.43. The GO-containing isotonic dispersions were stable with the following Z-ave results: GO1 421.1 nm, GO2 382.6 nm, GO3 440.2 nm and GO4 490.1 nm. The GO behavior was structure-dependent. Conclusion: BSA effectively stabilized four types of GO dispersions in an isotonic dispersion containing aggregating bivalent physiological cations.

引用

页数：30