Description and Genome Characterization of Three Novel Fungal Strains Isolated from Mars 2020 Mission-Associated Spacecraft Assembly Facility Surfaces-Recommendations for Two New Genera and One Species

被引：6

作者：

Chander, Atul Munish ^{[1
]}

Teixeira, Marcus de Melo ^{[2
,3
]}

Singh, Nitin K. ^{[1
]}

Williams, Michael P. ^{[1
]}

Simpson, Anna C. ^{[1
]}

Damle, Namita ^{[4
,5
]}

Parker, Ceth W. ^{[1
]}

Stajich, Jason E. ^{[6
]}

Mason, Christopher E. ^{[4
,5
]}

Torok, Tamas ^{[7
]}

Venkateswaran, Kasthuri ^{[1
]}

机构：

[1] CALTECH, Jet Prop Lab, Biotechnol & Planetary Protect Grp, M-S 89-2,4800 Oak Grove Dr, Pasadena, CA 91109 USA

[2] No Arizona Univ, Pathogen & Microbiome Inst, Flagstaff, AZ 86011 USA

[3] Univ Brasilia, Sch Med, BR-70910900 Brasilia, Brazil

[4] Weill Cornell Med, Dept Physiol & Biophys, New York, NY 10021 USA

[5] Weill Cornell Med, WorldQuant Initiat Quantitat Predict, New York, NY 10021 USA

[6] Univ Calif Riverside, Dept Microbiol & Plant Pathol, Riverside, CA 92521 USA

[7] Lawrence Berkeley Natl Lab, Ecol Dept, Berkeley, CA 94720 USA

来源：

JOURNAL OF FUNGI | 2023年 / 9卷 / 01期

关键词：

Aaosphaeria pasadenensis; Floridaphiala radiotolerans; fungi; genomics; Mars; 2020; mission; Pasadenomyces melaninifex; phylogenetic analysis; DATABASE; PLEOSPOROMYCETIDAE; COMMUNITIES; ASPERGILLUS; FAMILIES; BACTERIA;

D O I：

10.3390/jof9010031

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

National Aeronautics and Space Administration's (NASA) spacecraft assembly facilities are monitored for the presence of any bacteria or fungi that might conceivably survive a transfer to an extraterrestrial environment. Fungi present a broad and diverse range of phenotypic and functional traits to adapt to extreme conditions, hence the detection of fungi and subsequent eradication of them are needed to prevent forward contamination for future NASA missions. During the construction and assembly for the Mars 2020 mission, three fungal strains with unique morphological and phylogenetic properties were isolated from spacecraft assembly facilities. The reconstruction of phylogenetic trees based on several gene loci (ITS, LSU, SSU, RPB, TUB, TEF1) using multi-locus sequence typing (MLST) and whole genome sequencing (WGS) analyses supported the hypothesis that these were novel species. Here we report the genus or species-level classification of these three novel strains via a polyphasic approach using phylogenetic analysis, colony and cell morphology, and comparative analysis of WGS. The strain FJI-L9-BK-P1 isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) exhibited a putative phylogenetic relationship with the strain Aaosphaeria arxii CBS175.79 but showed distinct morphology and microscopic features. Another JPL-SAF strain, FJII-L3-CM-DR1, was phylogenetically distinct from members of the family Trichomeriaceae and exhibited morphologically different features from the genera Lithohypha and Strelitziana. The strain FKI-L1-BK-DR1 isolated from the Kennedy Space Center facility was identified as a member of Dothideomycetes incertae sedis and is closely related to the family Kirschsteiniotheliaceae according to a phylogenetic analysis. The polyphasic taxonomic approach supported the recommendation for establishing two novel genera and one novel species. The names Aaosphaeria pasadenensis (FJI-L9-BK-P1 = NRRL 64424 = DSM 114621), Pasadenomyces melaninifex (FJII-L3-CM-DR1 = NRRL 64433 = DSM 114623), and Floridaphiala radiotolerans (FKI-L1-BK-DR1 = NRRL 64434 = DSM 114624) are proposed as type species. Furthermore, resistance to ultraviolet-C and presence of specific biosynthetic gene cluster(s) coding for metabolically active compounds are unique to these strains.

引用

页数：24