Transcriptional profiling of early differentiation of primary human mesenchymal stem cells into chondrocytes

被引：4
|
作者：
Schwarzl, Thomas [1 ]
Keogh, Andrea [2 ]
Shaw, Georgina [3 ]
Krstic, Aleksandar [4 ]
Clayton, Elizabeth [2 ]
Higgins, Desmond G. [4 ,5 ]
Kolch, Walter [4 ,5 ]
Barry, Frank [3 ]
机构：
[1] European Mol Biol Lab EMBL, Meyerhofstr 1, D-69117 Heidelberg, Germany
[2] Natl Univ Ireland Galway, Regenerat Med Inst Remed, Biosci, Univ Rd, Galway, Ireland
[3] Natl Univ Ireland Galway, Regenerat Med Inst Remed, Biosci, Univ Rd, Galway, Ireland
[4] Univ Coll Dublin, Sch Med, Syst Biol Ireland SBI, Dublin, Ireland
[5] Univ Coll Dublin UCD, Conway Inst Biomol & Biomed Res, Dublin, Ireland
来源：
SCIENTIFIC DATA | 2023年 / 10卷 / 01期
基金：
爱尔兰科学基金会;
关键词：
STROMAL CELLS; PACKAGE; THERAPY;
D O I：
10.1038/s41597-023-02686-y
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Articular cartilage has only very limited regenerative capacities in humans. Tissue engineering techniques for cartilage damage repair are limited in the production of hyaline cartilage. Mesenchymal stem/stromal cells (MSCs) are multipotent stem cells and can be differentiated into mature cartilage cells, chondrocytes, which could be used for repairing damaged cartilage. Chondrogenesis is a highly complex, relatively inefficient process lasting over 3 weeks in vitro. Methods: In order to better understand chondrogenic differentiation, especially the commitment phase, we have performed transcriptional profiling of MSC differentiation into chondrocytes from early timepoints starting 15 minutes after induction to 16 hours and fully differentiated chondrocytes at 21 days in triplicates.
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页数：6
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