Development of ic-ELISA and colloidal cold-based immunochromatographic assay for red 2G detection in fruit drinks, red wine, and yoghurts

被引：7

作者：

Li, Xiaoling ^{[1
,2
]}

Xu, Xinxin ^{[1
,2
]}

Guo, Lingling ^{[1
,2
]}

Song, Shanshan ^{[1
,2
]}

Liu, Liqiang ^{[1
,2
]}

Zhu, Yingyue ^{[3
]}

Kuang, Hua ^{[1
,2
]}

Xu, Chuanlai ^{[1
,2
]}

Xu, Liguang ^{[1
,2
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Int Joint Res Lab Biointerface & Biodetect, Wuxi 214122, Jiangsu, Peoples R China

[3] Changshu Inst Technol, Sch Biotechnol & Food Engn, Changshu 215500, Jiangsu, Peoples R China

来源：

FOOD BIOSCIENCE | 2023年 / 52卷

关键词：

Monoclonal antibody; Red; 2G; Ic-ELISA; Hapten; Colloidal gold-based immunochromatographic assay; PERFORMANCE LIQUID-CHROMATOGRAPHY; SYNTHETIC FOOD COLORANTS; MONOCLONAL-ANTIBODY; DIODE-ARRAY; SENSITIVE DETERMINATION; GRAPHENE OXIDE; AZO DYES; IMMUNOASSAY; PRODUCTS; YELLOW;

D O I：

10.1016/j.fbio.2023.102445

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Red 2G (R2.i), a cheap industrial colorant, cannot be added to food. An anti-R2C nonoclonal antibody (mAb) was prepared by ininiunizing ptice with the conjugate of R2C hapten and protein, which based on the 1-ethy1-3(3-dimethylarninopropyl) carbodiimide (EDC) method. Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunocluomatographic assay (CG-ICA) rnetlods were used to determine R2G in fruit drinks, red wine, and yoghtwts. A standard curve of the developed ic-ELISA showed that the ICso of the anti-R2G rnAb was 1.02 ng/mL, and limit of detection value (LOZ) was 0.21 ng/mL. For the CGICA developed, the visual limit of detection values (vLOD) were 2 ng/ml. and cut-off valttes of 100 ng/ml. in sarnples. The results indicated that these two methods could be used to gttickly detec[ R2G in fruit drinks, red wine, and yoghtuts.

引用

页数：7