Liquid chromatography-mass spectrometry-based protocol to measure drug accumulation in tuberculosis and its host cell

被引：1

作者：

Ahn, Yong-Mo ^{[1
]}

Lavin, Richard C. ^{[2
,3
]}

Tan, Shumin ^{[2
,3
]}

Freundlich, Joel S. ^{[1
,4
,5
]}

机构：

[1] Rutgers State Univ, New Jersey Med Sch, Dept Pharmacol Physiol & Neurosci, Newark, NJ 07101 USA

[2] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA

[3] Tufts Univ, Grad Sch Biomed Sci, Grad Program Mol Microbiol, Boston, MA 02111 USA

[4] Rutgers State Univ, New Jersey Med Sch, Dept Med, Div Infect Dis, Newark, NJ 07103 USA

[5] Rutgers State Univ, Ruy V Lourenco Ctr Study Emerging & Reemerging Pat, New Jersey Med Sch, Newark, NJ 07103 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Chemistry; Immunology; Mass Spectrometry; Microbiology;

D O I：

10.1016/j.xpro.2022.101971

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The extent to which a drug accumulates in Mycobacterium tuberculosis (Mtb) and its host cell can affect treatment efficacy. We describe protocols measuring drug accumulation in Mtb, macrophages, and Mtb-infected macrophages. The method leverages drug extraction from the cellular lysate and drug-level quantification by liquid chromatography-mass spectrometry. The general methodology has broad applicability and can quantify drug accumulation in other cell types, while being extended to quantification of drug metabolites formed within the cell un-der study. For complete details on the use and execution of this protocol, please refer to Lavin et al. (2021).1

引用

页数：13

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