Cancer cells targeting H2O2-responsive MXene-integrated hyaluronic acid polymer dots coated sensor

We successfully developed a reactive oxygen species (ROS)-responsive electrochemical sensor based on a substrate coated with cancer cell-targeted hyaluronic acid polymer dot-integrated ROS-degradable MXene particles [MXene-PD(HA)] for identifying cancer conditions in vitro. Exposure to high H2O2 con-centration of cancer cells selectively controls the electroconductivity and photothermal conversion of the MXene-PD(HA) due to the involvement of oxidation reaction, changing MXene into TiO2. The resis-tance of the sensor increases with accumulation of H2O2, elevating the resistance from 225.4 +/- 62.39 k51 (0 mM) to 3418 +/- 39.22 k51 after treatment with 1 mM H2O2. In vitro treatment with CHO-K1 and HeLa cells adjusted the morphology of MXene-PD(HA)-coated surfaces and electrochemical sensing per-formance through the degradation of MXene by intracellular ROS. The sensor could differentiate normal and cancer cells by displaying an increase in resistance from 147.4 +/- 16.00 k51 (control) to 994.4 +/- 152.6 k51 (CHO-K1) and 2040 +/- 460.1 k51 (HeLa). Besides, the MXene-PD(HA) is uptaken after 1 h treatment for CHO-K1 cells and as early as 0.5 h for HeLa, displaying a rapid detection. In conclusion, the MXene-PD (HA) electrode exhibited the ability to distinguish between normal and cancer cells and showed promis-ing potential for the detection of tumor microenvironment. (c) 2022 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.