Sulfur Metabolism in Thiocapsa bogorovii BBS and the Role of HydSL Hydrogenase

被引：0
|
作者：
Khasimov, M. Kh. [1 ]
Petushkova, E. P. [1 ]
Serdyuk, O. P. [1 ]
Tsygankov, A. A. [1 ]
机构：
[1] Russian Acad Sci, Inst Basic Biol Problems, Pushchino Sci Ctr Biol Res, Pushchino 142290, Moscow Oblast, Russia
来源：
MICROBIOLOGY | 2023年 / 92卷 / SUPPL 1期
基金：
俄罗斯科学基金会;
关键词：
HydSL hydrogenase; Isp1; Isp2; elemental sulfur; sulfur reduction; sulfide; Thiocapsa bogorovii BBS; GENES; BACTERIUM;
D O I：
10.1134/S002626172360386X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The specific enzymatic reaction of sulfur reduction to hydrogen sulfide catalyzed by the HydSL hydrogenase of T. bogorovii BBS has so far been observed only in native cells. In this work, we were able to detect the hydrogen-dependent reaction H-2 + S-0 -> H2S + HS- + H+ in a cell homogenate of these bacteria. This was achieved by destroying the cells in an optimized buffer of high ionic strength. Further purification of the preparation led to a 28-fold increase in its specific activity.
引用
收藏
页码：S22 / S24
页数：3
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