Green synthesis and characterization of copper nano-particles with propolis and their potential inhibitory effect on uropathogenic E.coli

A current focus in nanotechnology and nanoscience is the green biosynthesis of nanoparticles (NPs) using biomaterials. Research on green methods for making metal oxide NPs is gaining momentum to safeguard the environment from the potential dangers associated with toxic chemicals. This study aimed to synthesize copper NPs (CuNPs) via propolis extraction, a novel application of nanoscience. The results of our study were characterized by UV-visible absorption spectra, XRD, FTIR, and SEM and then to figure out the antibacterial activity against UPEC strains. According to an FTIR examination, substances present in propolis extract may affect how the surface of the created NPs is modified. The propolis extract spectrum exhibited sharp peaks at 2924.45cm(-1), 1624.57cm(-1), and 1517.11cm(-1) to 1056.15cm(-1) and 3422 cm(-1), which might be due to C = O and C = C aromatic stretching frequencies. We found a characteristic peak at 385nm signifying the surface plasmon resonance (SPR) with propolis extract. Our results confirmed of the potential role of antibacterial activity by the green synthesized CuNPs. Significant MIC values were seen at 8.4 +/- 0.192 (1mg/ml) (P < 0.05). We also found significant biofilm inhibition (84%) and reduction in motility (92%) when compared to a positive control (Ampicillin). The qPCR study found fimH and fliC gene members to be downregulated to 35% and 66%. However, we could not find an effect on the BamA gene member. For clinical uses, such as drug delivery systems, medication formulation, and biomedical applications, the green production of CuNPs from propolis may be a suitable candidate.