Biodegradation of crystal violet dye by Saccharomyces cerevisiae in aqueous medium

Crystal violet (CV) is an azo dye with cationic nature, belonging to the triphenylmethane group. This study was designed to optimize CV removal by S. cerevisiae from aqueous solutions using BBD model. Harvested cells of S. cerevisiae were locally obtained from Iran Science and Technology Research Organization (ISTRO). The decolorization tests were performed in a laboratory container containing a 100 cc of reaction solution under different variables, including yeast dose (0.5-1.5 g/L), pH (4-10), dye concentration (10-100 mg/L), and the reaction time of 24 h. After stirring with a magnetic shaker at a speed of 400 rpm, 10 cc of each sample was taken and centrifuged at 4000 rpm for 10 min to separate the biomass from dye solution. Then, the su-pernatant was filtered and finally the remaining CV was measured by a spectrophotometer at lambda max 590 nm. After the optimization of the factors mentioned above, the removal efficiency of this dye was investigated at the reaction times of 0.5-72 h. The findings indicated that CV removal ranged from 53.92 to 84.99%. The maximum CV removal was obtained at the CV concentration of 100 mg/L, the pH of 7, and the S. cerevisiae dose of 1.5 g/L. The findings showed that the elimination efficiency is directly related to the initial CV concentration, pH, and S. cerevisiae dose. However, during the reaction time, the elimination efficiency decreased slightly. The findings of this study proved that CV can be removed from aqueous solutions with an easy and low-cost method based on the use of indigenous microorganisms.