Determination of 6-Nitro-7-(4'-Nitrophenyl)-5-Ethyl-4,7-Dihydropyrazolo[1,5-a]Pyrimidine-3-Carboxylate as a Potential Antitumor Agent by Voltammetry

被引:0
|
作者
Mozharovskaia, P. N. [1 ]
Ivoilova, A. V. [1 ]
Terekhova, A. A. [1 ]
Tsmokalyuk, A. N. [1 ]
Ivanova, A. V. [1 ]
Kozitsina, A. N. [1 ]
Rusinov, V. L. [1 ,2 ]
机构
[1] Ural Fed Univ, Ekaterinburg 620002, Russia
[2] Russian Acad Sci, Postovskii Inst Organ Synth, Ural Branch, Ekaterinburg 620137, Russia
关键词
quantitative substance analysis; square-wave voltammetry; pyrazolopyrimidines; nitro group; glassy-carbon electrode; antitumor agent; DRUG; NANOPARTICLES;
D O I
10.1134/S1061934823020089
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
6-Nitro-7-(4'-nitrophenyl)-5-ethyl-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carboxylate (1) is one of promising antitumor compounds exhibiting biological activity against type 2 casein kinase, which is currently considered as a promising target in chemotherapy. Using the method of cyclic voltammetry, it was shown that the electrochemical activity of compound 1 in a mixed solution of Tris-HCl and ethanol (1 : 1) at pH 7.5 on a glassy-carbon electrode is due to the electrochemical reduction of the nitro group conjugated with the phenyl ring. A method was developed for the determination of compound 1 by direct cathodic square-wave voltammetry. The linearity region of the corresponding calibration curve obtained in a solution of a mixture of Tris-HCl and ethanol (1 : 1) at pH 7.5 is 5-500 mg/L (R-2 = 0.988), the limit of detection is 0.8 mg/L, the limit of quantification is 2.4 mg/L. The accuracy of the developed procedure is close to 100%, the relative standard deviation is 1.4%.
引用
收藏
页码:378 / 384
页数:7
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