Nanoporous Quercetin-Loaded Silicon-Stabilized Hybrid Lipid Nanoparticles Alleviate Salt Stress in Tomato Plants

被引：7

作者：

Guerriero, Gea ^{[1
]}

Sutera, Flavia Maria ^{[2
]}

Hoffmann, Jonas ^{[1
]}

Leclercq, Celine C. ^{[1
]}

Planchon, Sebastien ^{[1
]}

Berni, Roberto ^{[1
]}

Hausman, Jean-Francois ^{[1
]}

Renaut, Jenny ^{[1
]}

Torabi-Pour, Nissim ^{[2
]}

Pennington, Holly Cherise ^{[2
]}

Ahmed, Mukhtar ^{[2
]}

Welsh, Michael ^{[2
]}

Dehsorkhi, Ashkan ^{[2
]}

Saffie-Siebert, Suzanne ^{[2
]}

机构：

[1] Luxembourg Inst Sci & Technol, Environm Res & Innovat Dept, L-4940 Hautcharage, Luxembourg

[2] SiSaf Ltd, Guildford GU2 7RE, England

来源：

ACS APPLIED NANO MATERIALS | 2023年

关键词：

silicon-stabilized hybrid lipid nanoparticles (sshLNPs); nano-biostimulants; salinity; Solanum lycopersicum; crop protection; ARABIDOPSIS-THALIANA; OXIDATIVE STRESS; LEAF SENESCENCE; DROUGHT STRESS; EXPRESSION; SEEDLINGS; GROWTH; GERMINATION; TOLERANCE; RESPONSES;

D O I：

10.1021/acsanm.2c05384

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The search for more effective methods to alleviate the negative effects of exogenous stresses in plants has inspired nano-technologies. It is in this context that the use of formulations containing nanoporous silicon-stabilized hybrid lipid nanoparticles acting as delivery systems of the flavonoid quercetin was investigated here. These formulations, referred to as phyto-couriers, proved their efficacy in protecting the important crop model Solanum lycopersicum "Micro-Tom" against salinity. Two phyto-courier formulations, GS1 and GS3, functionalized with 25 mg of quercetin and differing in the presence of trehalose were applied to salt-stressed tomato by foliar spraying. The shape and ordered structure of the palisade cells was completely compromised under salinity; however, the phyto-couriers preserved their elongated shape under abiotic stress. From a molecular point of view, some stress-responsive genes tended to decrease in expression in stressed leaves treated with the phyto-couriers. Shotgun proteomics confirmed the nano-biostimulant nature of the formulations: several proteins involved in cytoprotection against oxidative stress were more abundant in control leaves treated with the phyto-couriers. Proteins involved in chromatin remodeling were also more abundant in control leaves treated with the trehalose-containing GS3 formulation, a finding indicating a priming effect. Overall, the formulations showed promising results to enhance abiotic stress tolerance in a crop model through the mitigation of stress symptoms. The results presented proof of the stress-relieving properties of the silicon-stabilized hybrid lipid nanoparticles and are a proof-of-concept for the use of the phyto-courier nanotechnology in horticultural applications.

引用

页码：3647 / 3660

页数：14