A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3

被引：2

作者：

Guo, Yiwen ^{[1
,2
,3
,4
]}

Lin, Yun ^{[1
,2
,3
]}

Xie, Quan ^{[1
,2
,3
,4
]}

Zhang, Wenyuan ^{[1
,2
,3
,4
]}

Xu, Zhenqi ^{[1
,2
,3
,4
]}

Chao, Yifei ^{[1
,2
,3
,4
]}

Cao, Xudong ^{[1
,2
,3
,4
]}

Jiang, Huiru ^{[1
,2
,3
,4
]}

Li, Han ^{[1
,2
,3
,4
]}

Li, Tuofan ^{[1
,2
,3
,4
]}

Wan, Zhimin ^{[1
,2
,3
,4
]}

Shao, Hongxia ^{[1
,2
,3
,4
]}

Qin, Aijian ^{[1
,2
,3
,4
]}

Ye, Jianqiang ^{[1
,2
,3
,4
]}

机构：

[1] Yangzhou Univ, Coll Vet Med, Key Lab Jiangsu Prevent Vet Med, Key Lab Avian Prevent Med,Minist Educ, Yangzhou, Jiangsu, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China

[3] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Jiangsu, Peoples R China

[4] Yangzhou Univ, Inst Agr Sci & Technol Dev, Yangzhou, Jiangsu, Peoples R China

来源：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2023年 / 13卷

关键词：

DAdV-3; fiber-2; recombinant FAdV-4; CRISPR; Cas9; expression; replication; VACCINE;

D O I：

10.3389/fcimb.2023.1177866

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.

引用

页数：7

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