Single-Component Self-Healing Antibacterial Anti-Inflammatory Intracellular-Antioxidative Poly(itaconic acid-pluronic) Hydrogel for Rapid Repair of MRSA-Impaired Wound

The rapid healing and repair of multidrug-resistant bacteriainfectedwound is still a challenge in the field of wound surgery. It is aneffective strategy to develop multifunctional bioactive biomaterialswith anti-infection therapy and promoting tissue regeneration. However,most of conventional multifunctional wound healing biomaterials possessthe complicated composition and fabrication procedure, which may limittheir clinical transformation. Herein, we report a single-componentmultifunctional bioactive self-healing scaffold (itaconic acid-pluronic-itaconicacid) (FIA) with robust antibacterial antioxidant anti-inflammatorybioactivity for treating methicillin-resistant Staphylococcusaureus (MRSA) impaired wound. FIA scaffolds exhibitedthe temperature-responsive sol-gel behavior, good injectability,and broad-spectrum antibacterial activity (100% inhibition rate against S. aureus, E. coli, and MRSA). FIA possessed favorable hemocompatibility and cell compatibilityand even stimulated the cellular proliferation. FIA could efficientlyscavenge the intracellular reactive oxygen species (ROS), decreasethe inflammation factors expression, promote endotheliocyte migrationand blood tube formation, and reduce the M1 phenotype of macrophagesin vitro. FIA could significantly clear the MRSA infection, speedup the MRSA-infected wound healing and rapid formation of the normalepithelial layer and skin appendages. This work may provide a simpleand efficient multifunctional bioactive biomaterial strategy for overcomingthe challenge of the MRSA-impaired wound.