Establishing a mouse model of lung metastases using ultrasound-guided right heart ventricle injection

被引：0
|
作者：
Labora, Amanda [1 ]
Creech, Amanda [2 ]
Lee, Hailey [2 ]
Tabornal, Erin [2 ]
Radu, Caius [2 ]
Donahue, Timothy [1 ]
机构：
[1] Univ Calif Los Angeles, Dept Surg, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Mol & Med Pharmacol, Ahmanson Translat Imaging Div, Los Angeles, CA 90095 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金：
美国国家卫生研究院;
关键词：
Cancer; Model Organisms;
D O I：
10.1016/j.xpro.2023.102698
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report a technique to generate a murine model of lung metastases by selectively injecting tumor cells into the right heart ventricle under ultrasound guidance. First, we describe cell preparation and reference animal preparation as previously described. We then detail the technique using a previously described 3D-printed instrument stabilization device. Finally, we describe tumor growth surveillance using bioluminescent imaging. For complete details on the use and execution of this protocol, please refer to Labora et al.1
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页数：18
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