BRPF1 bridges H3K4me3 and H3K23ac in human embryonic stem cells and is essential to pluripotency

被引：1

作者：

Zhang, Cong ^{[1
,2
,3
,4
]}

Lin, Huaisong ^{[1
,3
,4
]}

Zhang, Yanqi ^{[1
,2
,3
,4
]}

Xing, Qi ^{[1
,2
,3
,4
]}

Zhang, Jingyuan ^{[1
,2
,3
,4
]}

Zhang, Di ^{[1
,2
,3
,4
]}

Liu, Yancai ^{[1
,7
]}

Chen, Qianyu ^{[1
,3
,4
]}

Zhou, Tiancheng ^{[1
,3
,4
]}

Wang, Junwei ^{[1
,3
,4
]}

Shan, Yongli ^{[1
,3
,4
]}

Pan, Guangjin ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Chinese Acad Sci, Hong Kong Inst Sci & Innovat, Guangzhou Inst Biomed & Hlth, Ctr Regenerat Med & Hlth,CAS Key Lab Regenerat Bio, Guangzhou 510530, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Ctr Cell Lineage & Dev, CAS Key Lab Regenerat Biol,Guangdong Prov Key Lab, Guangzhou 510530, Peoples R China

[4] Chinese Acad Sci, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangdong Prov Key Lab Stem Cell & Regenerat Med, Guangzhou 510530, Peoples R China

[5] Shandong First Med Univ & Shandong Acad Med Sci, Key Lab for Rare & Uncommon Dis Shandong Prov, Biomed Sci Coll, Jinan 250117, Shandong, Peoples R China

[6] Shandong First Med Univ & Shandong Acad Med Sci, Shandong Med Biotechnol Ctr, Jinan 250117, Shandong, Peoples R China

[7] Univ Sci & Technol China, Div Life Sci & Med, Hefei 230027, Anhui, Peoples R China

来源：

ISCIENCE | 2023年 / 26卷 / 02期

基金：

中国国家自然科学基金;

关键词：

HISTONE-H3; LYSINE-4; REGULATOR BRPF1; PHD FINGER; CHROMATIN; TRANSCRIPTION; POLYCOMB; ACETYLTRANSFERASES; RECOGNITION; EXPRESSION; LINES;

D O I：

10.1016/j.isci.2023.105939

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Post-translational modifications (PTMs) on histones play essential roles in cell fate decisions during development. However, how these PTMs are recognized and coordinated remains to be fully illuminated. Here, we show that BRPF1, a multi-histone binding module protein, is essential for pluripotency in human embryonic stem cells (ESCs). BRPF1, H3K4me3, and H3K23ac substantially co-occupy the open chromatin and stemness genes in hESCs. BRPF1 deletion impairs H3K23ac in hESCs and leads to closed chromatin accessibility on stemness genes and hESC differentiation as well. Deletion of the N terminal or PHD-zinc knuckle-PHD (PZP) module in BRPF1 completely impairs its functions in hESCs while PWWP module deletion partially impacts the function. In sum, we reveal BRPF1, the multi-histone binding module protein that bridges the crosstalk between different histone modifications in hESCs to maintain pluripotency.

引用

页数：23

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