Effects of edaravone on testicular torsion-detorsion injury in rats

被引:0
|
作者
Sahin, Yasar [1 ]
Ustuner, Evren [2 ]
Tutun, Hidayet [3 ]
Yildirim, Ebru [1 ]
Eroglu, Oguz [4 ]
Kurtdede, Efe [5 ]
Ozkabadayi, Yasin [6 ]
Guncum, Enes [1 ]
Kutluca, Kursat [7 ]
Bilge, Ahmet Bilgehan [1 ]
机构
[1] Kirikkale Univ, Fac Vet Med, Dept Pharmacol & Toxicol, Kirikkale, Turkiye
[2] Ankara Univ, Fac Med, Dept Radiol, Ankara, Turkiye
[3] Burdur Mehmet Akif Ersoy Univ, Fac Vet Med, Dept Pharmacol & Toxicol, Burdur, Turkiye
[4] Kirikkale Univ, Fac Vet Med, Dept Emergency Med, Kirikkale, Turkiye
[5] Ankara Univ, Fac Vet Med, Dept Biochem, Ankara, Turkiye
[6] Kirikkale Univ, Fac Vet Med, Dept Histol & Embryol, Kirikkale, Turkiye
[7] Hacettepe Univ, Fac Med, Ankara, Turkiye
关键词
cSMI; edaravone; ischemia/reperfusion; testicular torsion; testis; torsion-detorsion; ISCHEMIA-REPERFUSION INJURY; FREE-RADICAL SCAVENGER; OXIDATIVE STRESS; ISCHEMIA/REPERFUSION; DAMAGE; FAMILY; ASSAY;
D O I
10.1111/andr.13628
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Background and objective: This study aimed to assess the protective ability of edaravone on testicular torsion-detorsion injury in rats. Methods: Eighteen adult male Sprague-Dawley rats were randomly divided into three groups: Sham group (control, n = 6); testicular torsion/detorsion (T/D group, n = 6) and T/D+edaravone (T/D+E group, n = 6). The spermatic cords of rats of the T/D group and the T/D+E group were rotated 720(degrees) in a clockwise direction and maintained for 120 min in this torsion position. Around 90 min after the torsion, edaravone at a dose of 10 mg/kg dissolved in saline was administered IP to the T/D+E group. The testicle was counter-rotated to its normal position to allow reperfusion for 4 h. Left testes of each animal were excised 240 min after beginning of reperfusion. Oxidative stress markers (TAS, TOS, SOD, and MDA) and apoptotic pathways (Caspase 3, Caspase 8, Caspase 9, Bcl-2, and Bax,) were assessed by ELISA methods. Also, testicles were subjected to the histopathologic and ultrasound examinations. Results: Ultrasound imaging showed that edaravone reduced the surface area and increased vascularization in testicles with T/D (p < 0.0001, p < 0.05, respectively). Edaravone pretreatment markedly decreased the levels of MDA, TOS, Bcl-2, Bax, Caspase 3, Caspase 8, and Caspase 9 (p < 0.0001). Also, it increased significantly TAS levels (p < 0.0001) and reduced insignificantly SOD activity. Histopathologic examinations demonstrated that edaravone significantly attenuated the histological damage caused by T/D in testicles. Conclusion: Taken together, the findings indicate that pretreatment of edaravone has protective effect against testicular T/D injury.
引用
收藏
页码:1918 / 1927
页数:10
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