Enhanced photocatalytic degradation, antimicrobial and anticancer efficiency of mycosynthesized TiO2 nanoparticles using Pleurotus ostreatus mushroom extract: An eco-friendly approach

This study is one such study where an eco-friendly approach has been followed in biosynthesizing TiO2NPs from Pleurotus ostreatus mushroom extract. Therefore, we object to biosynthesizing TiO2NPs and characterize them morphologically using UV, XRD, FTIR, FESEM, EDX, HR-TEM, AFM, and GCMS. The UV-visible spectroscopy absorption of synthesized TiO2NPs has peaked at 335 nm. The antibacterial activity was investigated by both isolated bacteria pathogens (gram-positive and gram-negative). The maximum growth inhibition zone was noted in S. mutans (14.3 +/- 0.4), E. coli (14.1 +/- 0.3), and S. epidermidis (13.3 +/- 0.2). The in vitro cytotoxicity assay of TiO2NPs showed an IC50 value of 47.42 mu g/mL against MG-63 cancer cells. The photocatalytic degradation of methylene blue by TiO2NPs was investigated under UV-visible light irradiation. Due to the NPs tiny size and high dispersion, a rapid degradation (between 30 and 180 min) of almost 84.24% was accomplished. Even after the photocatalyst was used three times, there was no discernible difference in the TiO2NPs photocatalytic activity. The synthesized TiO2NPs successfully destroyed the Methylene Blue (MB) dye in an aqueous solution, promising wastewater dye remediation. Therefore, our research shows for the first time that biosynthesis of TiO2NPs using P. ostreatus extract can be an excellent outcome for emerging multipurpose and organic products.