Effect of Tumor Necrosis Factor-α on in vitro Prostaglandin Production in Buffalo Corpus Luteum

Background: Corpus luteum plays key role in embryonic survival. Prostaglandins are the important regulator controlling the life span of corpus luteum. The present study investigated the effect of various doses of TNFa on in vitro PGF2a and PGE2 production and expression profiling of PGFS and PGES mRNA in buffalo Corpus Luteum (CL). Methods: Buffalo ovaries with mid-luteal phase CL were collected from the abattoir and CL were enucleated from surrounding tissues. Corpus luteum were finely chopped, rinsed with HBSS (Hanks Balanced Salt Solution) medium; supplemented with gentamycin and 0.1% BSA and incubated at 37 & DEG;C for 1 hr in HBSS containing 0.1% collagenase. The cell suspension following filtration was washed by HBBS supplemented with gentamycin and 0.1% BSA (bovine serum albumin) and was treated with increasing doses of TNFa (0.1, 0.5 and 1.0 nM) and cultured at 38.5 & DEG;C, 5% CO2 level for 24 hr. Result: There was dose dependent increase in concentrations of PGF2a and PGE2 with increasing doses of TNFa. The PGFS (prostaglandin F synthase) mRNA expression increased with increasing doses of TNFa. However, there was decrease in PGES (prostaglandin E synthase) mRNA expression at 0.1 nM and 0.5 nM TNF & alpha; but PGES mRNA expression increased at 1.0 nM TNFa as compared to control. It can be concluded that TNFa may alter PGES and PGFS mRNA expression and prostaglandin secretion in buffalo CL.