The Western Lake Erie culture collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome

被引：10

作者：

Yancey, Colleen E. ^{[1
]}

Kiledal, E. Anders ^{[1
]}

Chaganti, Subba Rao ^{[2
]}

Denef, Vincent J. ^{[3
]}

Errera, Reagan M. ^{[4
]}

Evans, Jacob T. ^{[3
]}

Hart, Lauren N. ^{[5
,6
]}

Isailovic, Dragan ^{[7
,8
]}

James, William S. ^{[1
,9
]}

Kharbush, Jenan J. ^{[1
]}

Kimbrel, Jeffrey A.

Li, Wei

Mayali, Xavier

Nitschky, Helena ^{[1
]}

Polik, Catherine A. ^{[1
,10
]}

Powers, McKenzie A. ^{[1
,11
]}

Premathilaka, Sanduni H. ^{[7
,8
]}

Rappuhn, Nicole A. ^{[1
,9
]}

Reitz, Laura A. ^{[1
]}

Rivera, Sara R. ^{[1
]}

Zwiers, Claire C. ^{[1
,11
]}

Dick, Gregory J. ^{[1
,2
]}

机构：

[1] Univ Michigan, Earth & Environm Sci, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Cooperat Inst Great Lakes Res CIGLR, 4840 S State Rd, Ann Arbor, MI 48108 USA

[3] Univ Michigan, Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA

[4] Natl Ocean & Atmospher Adm NOAA, Great Lakes Environm Res Lab GLERL, 4840 S State Rd, Ann Arbor, MI 48108 USA

[5] Univ Michigan, Program Chem Biol, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Life Sci Inst, Ann Arbor, MI 48109 USA

[7] Univ Toledo, Dept Chem & Biochem, Toledo, OH 43606 USA

[8] Lawrence Livermore Natl Lab, Phys & Life Sci Directorate, Livermore, CA 94550 USA

[9] Univ Michigan, Sch Environm & Sustainabil, Ann Arbor, MI 48109 USA

[10] Univ Minnesota, Ecol Evolut & Behav, St Paul, MN 55108 USA

[11] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA

来源：

HARMFUL ALGAE | 2023年 / 126卷

基金：

美国海洋和大气管理局; 美国国家科学基金会;

关键词：

CyanoHABs; Microcystis; Western Lake Erie; Metagenomics; Phycosphere; Microcystin; HARMFUL ALGAL BLOOMS; OXIDATIVE STRESS; GREEN-ALGAE; CYANOBACTERIUM; EVOLUTION; CO2; QUANTIFICATION; BIOSYNTHESIS; PLANKTOTHRIX; DEGRADATION;

D O I：

10.1016/j.hal.2023.102440

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Cyanobacterial harmful algal blooms (cyanoHABs) dominated by Microcystis spp. have significant public health and economic implications in freshwater bodies around the world. These blooms are capable of producing a variety of cyanotoxins, including microcystins, that affect fishing and tourism industries, human and environ-mental health, and access to drinking water. In this study, we isolated and sequenced the genomes of 21 pri-marily unialgal Microcystis cultures collected from western Lake Erie between 2017 and 2019. While some cultures isolated in different years have a high degree of genetic similarity (genomic Average Nucleotide Identity >99%), genomic data show that these cultures also represent much of the breadth of known Microcystis diversity in natural populations. Only five isolates contained all the genes required for microcystin biosynthesis while two isolates contained a previously described partial mcy operon. Microcystin production within cultures was also assessed using Enzyme-Linked Immunosorbent Assay (ELISA) and supported genomic results with high con-centrations (up to 900 mu g L-1) in cultures with complete mcy operons and no or low toxin detected otherwise. These xenic cultures also contained a substantial diversity of bacteria associated with Microcystis, which has become increasingly recognized as an essential component of cyanoHAB community dynamics. These results highlight the genomic diversity among Microcystis strains and associated bacteria in Lake Erie, and their potential impacts on bloom development, toxin production, and toxin degradation. This culture collection significantly increases the availability of environmentally relevant Microcystis strains from temperate North America.

引用

页数：15