Design, Synthesis and Characterization of Deoxycholic Acid-chalcone Conjugates as Antioxidant Agents

The present article reports the synthesis, characterization, and antioxidant activity of the chalcone-deoxycholic acid conjugates. The structures of the synthesised bile acid-chalcone conjugates have been determined using 1H and 13C NMR spectroscopy. According to the results of the DPPH experiment, compounds 5e (3, 4, 5-methoxy; IC50; 15.04 mu g /ml) and 5f (4-hydroxy group; IC50; 11.73 mu g /ml) had higher antioxidant activity than the control compound ascorbic acid (IC50; 20.72 mu g /ml). The best conjugate was found to be 5f in the DPPH test (IC50: 11.73 mu g /ml; 4-hydroxy group), while the best conjugate in the ABTS assay was found to be 5g (IC50: 67.97 mu g /ml; pyridine group). It is straightforward to synthesize a huge library of bile acid-derived compounds and employ them in comprehensive structure-activity relationship investigations to identify the compounds with the best antioxidant activity.