Progress on the Stereocontrolled Synthesis of Phosphorothioate Oligonucleotides

Phosphorothioate(PS) oligonucleotides have found a wide range of applications in biochemical mecha-nism studies, biomedicine, materials and related fields. The stereo configuration of PS has a remarkable effect on the biochemical properties of PS nucleic acids, which has aroused wide interest in developing methods for efficient and stereoselective synthesis of PS oligonucleotides in the past 30 years. This review summarized the methods for stereo -controlled synthesis of PS oligonucleotides, with a focus on the research progress in the past decade. The advantages and disadvantages of different methods were comparatively analyzed. Finally, the prospect of stereocontrolled synthe-sis of PS oligonucleotides was briefly discussed.