Identification of Oxidative-Stress-Reducing Plant Extracts from a Novel Extract Library-Comparative Analysis of Cell-Free and Cell-Based In Vitro Assays to Quantitate Antioxidant Activity

被引：7

作者：

Heckmann, Mara ^{[1
]}

Stadlbauer, Verena ^{[1
,2
]}

Drotarova, Ivana ^{[1
]}

Gramatte, Theresa ^{[1
,2
]}

Feichtinger, Michaela ^{[1
]}

Arnaut, Verena ^{[1
]}

Atzmueller, Stefanie ^{[1
]}

Schwarzinger, Bettina ^{[1
,2
]}

Roehrl, Clemens ^{[1
]}

Blank-Landeshammer, Bernhard ^{[1
,2
]}

Weghuber, Julian ^{[1
,2
]}

机构：

[1] Univ Appl Sci Upper Austria, Ctr Excellence Food Technol & Nutr, Stelzhamerstr 23, A-4600 Wels, Austria

[2] FFoQSI GmbH, Austrian Competence Ctr Feed & Food Qual, Safety & Innovat, Technopk 1D, A-3430 Tulln, Austria

来源：

ANTIOXIDANTS | 2024年 / 13卷 / 03期

关键词：

oxidative stress; plant extracts; antioxidant; extract library; screening; ROS; NO; NITRIC-OXIDE; CHEMICAL-COMPOSITION; NATURAL-PRODUCTS; INFLAMMATION; BIOACTIVITY; CAPACITY; LEAVES;

D O I：

10.3390/antiox13030297

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous underexplored plant species are believed to possess considerable potential in combating oxidative stress and its associated health impacts, emphasizing the need for a comprehensive methodological screening approach to assess their antioxidant capacity. This study investigated 375 plant extracts, utilizing both cell-free and cellular methods to evaluate their antioxidant properties. Target-based antioxidant capacity was evaluated by the total phenolic content (TPC) and ferric reducing antioxidant power (FRAP) assays. Cell-based assays employed the H2DCF-DA probe to measure reactive oxygen species (ROS) levels and the Griess assay to quantify nitric oxide (NO) levels in stressed Caco-2 and RAW264.7 cells, respectively. The highest TPC and FRAP values were found in extracts of Origanum vulgare and Fragaria x ananassa leaves. Several plant extracts significantly reduced stress-induced ROS or NO levels by at least 30%. Distinctive selectivity was noted in certain extracts, favoring the significant reduction of NO (e.g., Helianthus tuberosus extract), of ROS (e.g., Prunus domestica subsp. Syriaca extract), or of both (e.g., Fragaria x ananassa leaf extract). A strong correlation between TPC and FRAP values and moderate correlations between the results of the cell-free and cell-based assays were evident. These findings highlight the great antioxidant potential of underexplored plant extracts and the diversity of the underlying mechanisms, emphasizing the importance of a multifaceted approach for a comprehensive assessment.

引用

页数：15