While lecturers' teaching practices continue to be a focal point of English-medium In-struction (EMI) research, contrastive studies between EMI and L1 lectures remain extremely scarce. The present study addresses this research gap by analyzing five sets of matching L1 and English-medium lectures given in different disciplines at three Italian universities. Each set of lectures is given by the same lecturer, about the same topic. Thus, the study, closely examines the lectures' content in order to investigate which changes, if any, accompany the linguistic shift from L1 to English-medium teaching. The investigation focuses mainly on quantity and organization of content; nonetheless, other variables (such as speech rate, questions, language alternation), which can play a relevant role towards the organization of the lecture, are also considered. The analysis reveals a high correspondence of the core topics addressed in the lecturers; however, significant differences in the way such knowledge is conveyed are also observed. Such differences are grouped into three categories: differences in content quantity, differences in content selection and differences in rhetorical devices used.(c) 2023 The Author. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).