Mechanistic insight for improving butenyl-spinosyn production through combined ARTP/UV mutagenesis and ribosome engineering in Saccharopolyspora pogona

被引:8
|
作者
Zhao, Xueli [1 ]
Hussain, Muhammad Hammad [1 ]
Mohsin, Ali [1 ]
Liu, Zebo [1 ]
Xu, Zhixian [1 ]
Li, Zhanxia [2 ]
Guo, Weiqun [3 ]
Guo, Meijin [1 ]
机构
[1] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Dept Resp Med, Shanghai Peoples Hosp 6, Sch Med, Shanghai, Peoples R China
[3] Acad Natl Food & Strateg Reserves Adm, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
Saccharopolyspora pogona; butenyl-spinosyn; ARTP/UV mutagenesis; ribosome engineering; fermentation process; multi-omics analysis; HIGH-YIELD; UV; BIOSYNTHESIS; IMPROVEMENT;
D O I
10.3389/fbioe.2023.1329859
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Butenyl-spinosyn is a highly effective, wide-spectrum and environmentally-friendly biological insecticide produced by Saccharopolyspora pogona. However, its scale-up is impeded due to its lower titer in wild-type strains. In this work, ARTP/UV mutagenesis and ribosome engineering were employed to enhance the butenyl-spinosyn production, and a stable mutant Saccharopolyspora pogona aG6 with high butenyl-spinosyn yield was successfully obtained. For the first time, the fermentation results in the 5 L bioreactor demonstrated that the butenyl-spinosyn produced by mutant Saccharopolyspora pogona aG6 reached the maximum value of 130 mg/L, almost 4-fold increase over the wild-type strain WT. Furthermore, comparative genomic, transcriptome and target metabolomic analysis revealed that the accumulation of butenyl-spinosyn was promoted by alterations in ribosomal proteins, branched-chain amino acid degradation and oxidative phosphorylation. Conclusively, the proposed model of ribosome engineering combined with ARTP/UV showed the improved biosynthesis regulation of butenyl-spinosyn in S. pogona.
引用
收藏
页数:14
相关论文
共 7 条
  • [1] The PurR family transcriptional regulator promotes butenyl-spinosyn production in Saccharopolyspora pogona
    Li, Xinying
    Wang, Jingnan
    Su, Chang
    Guo, Chao
    Xu, Zhouqin
    Wang, Kehui
    Pang, Jian
    Lv, Bo
    Wang, Chao
    Li, Chun
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2025, 109 (01)
  • [2] Promoting Butenyl-spinosyn Production Based on Omics Research and Metabolic Network Construction in Saccharopolyspora pogona
    Rang, Jie
    Cao, Li
    Shuai, Ling
    Liu, Yang
    Zhu, Zirong
    Xia, Ziyuan
    Jin, Duo
    Sun, Yunjun
    Yu, Ziquan
    Hu, Shengbiao
    Xie, Qingji
    Xia, Liqiu
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022, 70 (11) : 3557 - 3567
  • [3] Addition of vegetable oil to enhance the biosynthesis of butenyl-spinosyn in a high production strain Saccharopolyspora pogona
    Xu, Zhouqin
    Li, Xinying
    Guo, Chao
    Su, Chang
    Wang, Chao
    PLOS ONE, 2025, 20 (03):
  • [4] Exploring a High-Efficiency Genetic Transformation System for Engineering Saccharopolyspora pogona ASAGF58 To Improve Butenyl-Spinosyn Production
    Pang, Jian
    Li, Xinying
    Guo, Chao
    Xu, Zhouqin
    Wang, Jingnan
    Lv, Bo
    Wang, Chao
    Li, Chun
    ACS AGRICULTURAL SCIENCE & TECHNOLOGY, 2023, 3 (02): : 203 - 210
  • [5] Enhancement of angucycline production by combined UV mutagenesis and ribosome engineering and fermentation optimization inStreptomyces dengpaensisXZHG99T
    Li, Yumei
    Li, Jiyu
    Ye, Zhengmao
    Lu, Lingchao
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2021, 51 (02): : 173 - 182
  • [6] Strain improvement by combined UV mutagenesis and ribosome engineering and subsequent fermentation optimization for enhanced 6′-deoxy-bleomycin Z production
    Zhu, Xiangcheng
    Kong, Jieqian
    Yang, Hu
    Huang, Rong
    Huang, Yong
    Yang, Dong
    Shen, Ben
    Duan, Yanwen
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 102 (04) : 1651 - 1661
  • [7] Strain improvement by combined UV mutagenesis and ribosome engineering and subsequent fermentation optimization for enhanced 6′-deoxy-bleomycin Z production
    Xiangcheng Zhu
    Jieqian Kong
    Hu Yang
    Rong Huang
    Yong Huang
    Dong Yang
    Ben Shen
    Yanwen Duan
    Applied Microbiology and Biotechnology, 2018, 102 : 1651 - 1661