Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next -Generation Sequencing (NGS) Method

被引：0
|
作者：
Ehya, Farveh [1 ]
Kalantari, Sima [2 ,3 ]
Bandehpour, Mojgan [4 ,5 ]
Kazemi, Bahram [4 ,5 ]
机构：
[1] Tarbiat Modares Univ, Dept Biotechnol, Tehran, Iran
[2] Univ Tehran Med Sci, Shariati Hosp, Digest Dis Res Inst, Digest Oncol Res Ctr, Tehran, Iran
[3] Universal Sci Educ & Res Network USERN, Regenerat Med Grp REMED, Tehran, Iran
[4] Shahid Beheshti Univ Med Sci, Cellular & Mol Biol Res Ctr, Tehran, Iran
[5] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Biotechnol, Tehran, Iran
来源：
IRANIAN JOURNAL OF PARASITOLOGY | 2023年 / 18卷 / 03期
关键词：
Lizard leishmania; RNA sequencing; Transcriptome; Next generating sequencing; TISSUE-PLASMINOGEN ACTIVATOR; PTERIDINE REDUCTASE 1; GENOMIC ORGANIZATION; MOLECULAR-CLONING; GENE ORGANIZATION; EXPRESSION; CHROMOSOME-1;
D O I：
暂无
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Background: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far.Methods: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. Leishmania promastigotes were cultured in RPMI1640 culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and<br />quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China).Results: The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania.Conclusion: Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system
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页码：362 / 368
页数：7
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